Protein Info for HP15_3676 in Marinobacter adhaerens HP15

Annotation: glucosamine-fructose-6-phosphate aminotransferase, isomerizing

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 610 (609 residues), 870.6 bits, E=3e-266
• PF13230: GATase_4
  • amino acids 53 to 129 (77 residues), 25.4 bits, E=1.3e-09
• PF13522: GATase_6
  • amino acids 66 to 185 (120 residues), 79.9 bits, E=3.5e-26
• PF13537: GATase_7
  • amino acids 86 to 196 (111 residues), 60.8 bits, E=2.5e-20
• PF01380: SIS
  • amino acids 291 to 418 (128 residues), 119.3 bits, E=2e-38
  • amino acids 464 to 592 (129 residues), 85.7 bits, E=5e-28

Best Hits

Swiss-Prot: 66% identical to GLMS_PSEPK: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 91% identity to maq:Maqu_3872)

MetaCyc: 61% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See E4PHD9 at UniProt or InterPro

Protein Sequence (610 amino acids)

>HP15_3676 glucosamine-fructose-6-phosphate aminotransferase, isomerizing (Marinobacter adhaerens HP15)
MCGIVGAVSERDVQGILLEGLRRLEYRGYDSAGMAVIGGDHSVQRAREVGKVAALAEAME
ANPLAGHLGIAHTRWATHGEPSQINAHPHMSGDRLAIVHNGIIENYQELREELRADGFEF
TSQTDTEVVAHLIEKHYRTLGNLHDSVRSAIARLRGAYALAVVHADEPEHLVVCREGSPL
VIGVGIGENFIASDQLALLPVTDRFMFLEEGDIADIRKDGIAILDRDGKAVEREVTRFEH
GADSADKGEYRHFMLKEIYEQPKVIKATMEGRVTRTRVLEQALGTEAANLLENVRHVQII
ACGTSYHAGMVARYWIEELAGVACSVEVASEFRYRKHVIQQDTLFLCISQSGETADTLAA
LRQAKKAGFRAALAICNVPGSSLVRESDLVIMTQAGPEIGVASTKAFTTQLTALLIFTLA
LARHNGLPEEQEAEIVEALHLVPGQVSDVLALDGEIAEMSKAFMDKNHSLFLGRGSQFPV
ALEGALKLKEISYIHAEAYPAGELKHGPLALVDSEMPVVTVAPNNDLVEKLKSNLEEVRA
RGGELFVFADKKADVKAEEGLHVMQIPSVHPITAPIVYTVPLQLLSYHVAVLKGTDVDQP
RNLAKSVTVE