Protein Info for Psest_3785 in Pseudomonas stutzeri RCH2

Annotation: Glycosidases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 511 PF00128: Alpha-amylase" amino acids 32 to 360 (329 residues), 327 bits, see alignment E=1.9e-101 PF11941: DUF3459" amino acids 420 to 505 (86 residues), 40.3 bits, see alignment E=3.5e-14

Best Hits

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 93% identity to psa:PST_0490)

Predicted SEED Role

"Alpha-glucosidase (EC 3.2.1.20)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GS96 at UniProt or InterPro

Protein Sequence (511 amino acids)

>Psest_3785 Glycosidases (Pseudomonas stutzeri RCH2)
MSETTRTPWWKGATVYQIYPRSFADSNGDGIGDLNGVLRHLDHLQQLGVDALWLSPIFRS
PMADAGYDISDYCDIDPLFGSLADIDRLIAEAHARNIRVLLDFVPNHTSDEHPWFVESRS
SRDNPKRDWYIWRDQPNNWRAALGAGSAWTWDEHTQQYYLHLFLAKQPDLNWRNPEVVEA
MHDVLRFWLDRGIDGFRIDVIHCTGKDLSFADDPRCQAGQPLSDFNDQPYSHEVLRGLRK
LVDSYPGDRMLVGEVNIRSTERVLQYYGAGDELHLAFNFNPLDAPWDPVLFRTCIREVEH
WLQPAGAWPTWVLSNHDNVRQRSRYRGSERAARAAAVLLLTLRGTPFIYQGEELGLEDAH
IRDEARIDPGGRDGCRAPIPWQAEPPHGWQGAEPWLPFPPDAAELAAERQTGAANSIFAL
YQRLLAARKASPALHHGDFEELASHPEVLAYRRQHGDDCRLVCINFSEQPHAHPHAGDWH
VEIASDGVGENAPYNGTLNPGQAVVLRPMEN