Protein Info for Psest_3778 in Pseudomonas stutzeri RCH2

Annotation: ATP-dependent DNA helicase Rep

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 669 TIGR01074: ATP-dependent DNA helicase Rep" amino acids 3 to 668 (666 residues), 1079.3 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 4 to 266 (263 residues), 305.1 bits, see alignment E=1.6e-94 PF13245: AAA_19" amino acids 8 to 249 (242 residues), 83.7 bits, see alignment E=4.2e-27 PF13361: UvrD_C" amino acids 271 to 613 (343 residues), 247.1 bits, see alignment E=1.1e-76

Best Hits

KEGG orthology group: K03656, ATP-dependent DNA helicase Rep [EC: 3.6.4.12] (inferred from 95% identity to pfv:Psefu_0717)

Predicted SEED Role

"ATP-dependent DNA helicase Rep" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNC3 at UniProt or InterPro

Protein Sequence (669 amino acids)

>Psest_3778 ATP-dependent DNA helicase Rep (Pseudomonas stutzeri RCH2)
MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAYLVQQCGIQARHIVAMTFTNKA
AREMKERVGSLLKGAEARGLTVSTFHNLGMNIIRKEYARMGYKPGFSIFDDGDIKALLTD
IMQKEYSGDDGADEIKNYIDSWKNDLILPDEALANARNPKEQTAAIVYLHYQRTLKAYNA
VDFNDLILLPVKLFQEHADILEKWQNRIRYLLVDEYQDTNASQYLLVKLLVGMRNQFTVV
GDDDQSIYAWRGARPENLMLLKEDYPSLKVVMLEQNYRSTSRILKCANILIANNPHVFEK
QLWSEMGHGDEIRVIRTRNEDAECERVALEILTEHLRTQRPYSDFAILYRGNYQAKLMEL
KLQHHQIPYRLSGGTSFFARQEVKDLMSYFRLLVNPDDDNAFLRVINVPRREIGSTTLEK
LGNYASERGISMYAAADEIGLGAHLDSRYAERLARFKHWMDNVRQQCVVNEPIAAIRSMV
MDIDYENWLRQNASSDKVADARMGNVWFLVDALKNTLDKDEDGDMTIEDAIGKLVLRDML
ERQQEEEEGAEGVQMMTMHASKGLEFPSVYIIGFEEEILPHRSSIESDTIEEERRLAYVG
ITRAKRNLALTFAAKRKQYGEVIDCTPSRFLDELPPEDLVWEGQEDAPVEVKAARGNDAL
AAMRAMLKR