Protein Info for Psest_3745 in Pseudomonas stutzeri RCH2

Annotation: Uncharacterized enzyme of heme biosynthesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 42 to 73 (32 residues), see Phobius details TIGR00540: heme biosynthesis-associated TPR protein" amino acids 6 to 394 (389 residues), 328.3 bits, see alignment E=2.8e-102 PF07219: HemY_N" amino acids 27 to 133 (107 residues), 91.1 bits, see alignment E=7.1e-30 PF14559: TPR_19" amino acids 131 to 197 (67 residues), 26.6 bits, see alignment E=9.1e-10

Best Hits

KEGG orthology group: K02498, HemY protein (inferred from 91% identity to psa:PST_0527)

Predicted SEED Role

"Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQC5 at UniProt or InterPro

Protein Sequence (406 amino acids)

>Psest_3745 Uncharacterized enzyme of heme biosynthesis (Pseudomonas stutzeri RCH2)
MKRLALVFILLLAIAGFLGWAISQSAGYVLITYDQFRYESSFWIFLALVGCLWLLAMVVH
WLLGVLQVSGALVNPWSRRHRARRVENASRKGLRELAEGQWGPALAHLQLAAEKDRQPLV
HYLGAARAANELGEYAQSDELLHKAREREPEAALAIGLTQAQLQIARGQYVEARASLSEL
QNDHPRHPYVLTLLQQLYVQLEDWAALCRLLPELRKHRVLPPARLSELEVLAWTAAVEQA
GQPSEVAAEAGIEALNQRWQTVPSALRSEPLVVRAYADGLSRLGAQPKAEEVLYAAIKRQ
FDDRLVERYGRVQGRDPARQLAHAEGWLKDHPQNPVLLLALGRLSLRNELWGKARDYLEA
SLRFDHRPETCAELARLLAQLGDTENSNRLFQQGVGLLGRNLPATL