Protein Info for HP15_3619 in Marinobacter adhaerens HP15

Annotation: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 TIGR03140: alkyl hydroperoxide reductase subunit F" amino acids 1 to 518 (518 residues), 803.5 bits, see alignment E=3.7e-246 PF13192: Thioredoxin_3" amino acids 125 to 195 (71 residues), 32.2 bits, see alignment E=2.7e-11 PF07992: Pyr_redox_2" amino acids 214 to 504 (291 residues), 161.7 bits, see alignment E=8.2e-51 PF13738: Pyr_redox_3" amino acids 261 to 487 (227 residues), 55.1 bits, see alignment E=2.2e-18 PF00070: Pyr_redox" amino acids 357 to 429 (73 residues), 47.6 bits, see alignment E=6.1e-16

Best Hits

Swiss-Prot: 70% identical to AHPF_PSEAE: Alkyl hydroperoxide reductase subunit F (ahpF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 89% identity to maq:Maqu_3837)

MetaCyc: 64% identical to alkyl hydroperoxide reductase, AhpF component (Escherichia coli K-12 substr. MG1655)
RXN-8506 [EC: 1.5.1.37]; R4-RXN [EC: 1.5.1.37, 1.11.1.26]

Predicted SEED Role

"Alkyl hydroperoxide reductase protein F (EC 1.6.4.-)" in subsystem Thioredoxin-disulfide reductase (EC 1.6.4.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.4.-

Use Curated BLAST to search for 1.11.1.26 or 1.5.1.37 or 1.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PGS4 at UniProt or InterPro

Protein Sequence (528 amino acids)

>HP15_3619 FAD-dependent pyridine nucleotide-disulfide oxidoreductase (Marinobacter adhaerens HP15)
MLDANIKEQLKAYMDKLQQPIELVAAYDDSKKSQELRELLEELEPMSSKISLRTEESNDV
RRPSFAINRVGTDIGVRFAGIPMGHEFTSLVLALLQVGGHPSKASQDVIEQIQSLEGRFE
FETYFSLSCQNCPDVVQALNLMSVLNPNITNTSIDGALFQDEVEQREVMAVPSVYMNGEP
WGQGRMTLEEIVAKLDTSSSEKDAEKINQKDAFEVLIIGGGPAGASAAIYAARKGISTGI
AAERFGGQVADTMGIENLISVPYTEGPKLVAAMEQNVKEYDVDIMNMQRAEKLIPASRPG
MLHQVRLENGATLKARSLVLSTGARWRQMGVPGEEEYRNKGVAYCPHCDGPLFKGKRVAV
IGGGNSGVEAAIDLAGIVGHVTLIEFGAEMRADEVLQKKLRSLKNVEIITSGQTTEITGE
NGKVNGLNYTDRTSGEQHHISLEGVFVQIGLVPNTEWLKGDIELSQHGEIIVNDRNETSI
PGVFAAGDATTVPYKQIVISMGEGSKAALSAFDFLIRNSVDESEDEAA