Protein Info for Psest_3724 in Pseudomonas stutzeri RCH2

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 740 PF17764: PriA_3primeBD" amino acids 8 to 105 (98 residues), 91.5 bits, see alignment E=7.3e-30 PF04851: ResIII" amino acids 202 to 364 (163 residues), 60.6 bits, see alignment E=5.5e-20 PF00270: DEAD" amino acids 207 to 369 (163 residues), 75.5 bits, see alignment E=1.3e-24 TIGR00595: primosomal protein N'" amino acids 226 to 737 (512 residues), 613.8 bits, see alignment E=1.2e-188 PF18319: PriA_CRR" amino acids 450 to 476 (27 residues), 41.5 bits, see alignment (E = 3e-14) PF00271: Helicase_C" amino acids 510 to 593 (84 residues), 25.9 bits, see alignment E=3e-09 PF18074: PriA_C" amino acids 637 to 736 (100 residues), 87.3 bits, see alignment E=3e-28

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 93% identity to psa:PST_0549)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQA8 at UniProt or InterPro

Protein Sequence (740 amino acids)

>Psest_3724 primosomal protein N' (Pseudomonas stutzeri RCH2)
MPSCILRLALPSPLRRLFDYLPPAGVSRAALQPGVRLRVPFGRREVVGILVELSDQSEVP
VEKLKPALELLDAKPPLPAPLFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAEARQER
YWRLAEGASPEDPRLARAPKQREALKAIAQHAHGLPHALLSKLQLNKDSLDLLLEKGLVH
IESRRSKPSRGHSGSWLLQAELTLNAQQRAAFEAVSAGLGGFQAFLLAGVTGSGKTEVYL
QLIHRVLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSNVNDRDRLDAWLAARDG
EADIIIGTRSALFTPMKNPGLIIIDEEHDASYKQQEGLRYHARDLALVRARQENLPIVLG
SATPSLESLHNAHSGRYALLKLTQRAGNAQSPRFLRLDVKSRPLDAGISGPLQQAIGQTL
AAGQQVLIYLNRRGFAPTLLCHDCGWLSQCPRCDARMTLHQRHNELRCHHCGHVERPPRN
CPDCGKVDLRPVGAGTERAEERLGILFPDYPVLRIDRDSTARKGAMEQMIGTINRGEPCL
LVGTQMLAKGHHFPRVTLVAILDADGGLFSADFRASERMAQLIVQVAGRAGRAEEPGKVI
IQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFSHLALLRAEAQKPGQAEAFLDD
ACSLAEQLLEQLQLNGVELLGPVPAPMERRAGRYRAQLLLQGNARATLHRLMDIWTPTLE
QLPGSRAVRWSLDVDPIDLF