Protein Info for Psest_3721 in Pseudomonas stutzeri RCH2
Annotation: Malic enzyme
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 77% identical to MAEB_HALED: NADP-dependent malic enzyme (maeB) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)
KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 99% identity to psa:PST_0550)Predicted SEED Role
"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)
MetaCyc Pathways
- gluconeogenesis I (11/13 steps found)
- C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
- methylgallate degradation (2/6 steps found)
- protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
- superpathway of vanillin and vanillate degradation (3/10 steps found)
- syringate degradation (3/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Pyruvate metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GN77 at UniProt or InterPro
Protein Sequence (422 amino acids)
>Psest_3721 Malic enzyme (Pseudomonas stutzeri RCH2) MTDLKTAALEYHAQPRPGKLSVELTKPTATARDLSLAYSPGVAEPVREIARDAELAYRYT GKGNLVAVISDGTAILGLGNLGPLASKPVMEGKGVLFKRFAGVDVFDIEVDAESPQAFID TVKRISITFGGINLEDIKAPECFEIERALIEQCDIPVFHDDQHGTAIVTAAGMLNALEIA GKTLEQAKIVCLGAGAAATSCMKLLVSMGAKIENIFMIDRKGVIHAGRDDLNQYKAVFAH ETDKRTLDDALEGADVFVGLSGANLLSPDGLKRMAANPVVFACSNPDPEISPELAHATRS DVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAKRINEEMKIAAAIALRDLAKLPVPAEV AAAYGVDSLEFGREYIIPKPMDPRLITLVSDAVAKAAIESGVATLPYPSHYPLQSVHDVF NG