Protein Info for Psest_3720 in Pseudomonas stutzeri RCH2

Annotation: penicillin-binding protein, 1A family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 809 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00912: Transgly" amino acids 56 to 229 (174 residues), 218.9 bits, see alignment E=5.3e-69 TIGR02074: penicillin-binding protein, 1A family" amino acids 65 to 737 (673 residues), 601.8 bits, see alignment E=6.6e-185 PF17092: PCB_OB" amino acids 316 to 420 (105 residues), 83.3 bits, see alignment E=3.4e-27 PF00905: Transpeptidase" amino acids 424 to 728 (305 residues), 106.4 bits, see alignment E=2.6e-34

Best Hits

KEGG orthology group: K05366, penicillin-binding protein 1A [EC: 2.4.1.- 3.4.-.-] (inferred from 72% identity to ppf:Pput_4957)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-, 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.- or 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQY2 at UniProt or InterPro

Protein Sequence (809 amino acids)

>Psest_3720 penicillin-binding protein, 1A family (Pseudomonas stutzeri RCH2)
MRFLKFLLWSCFAVFCALLLSISGAFLYLSPNLPSVDSLRSIQLQIPLRVYSADDKLIAE
FGEMRRSPIRFEDIPQEFIQALLAAEDDNFANHYGVDITGLMRAATQLLKSGQIQTGGST
ITMQVAKNYFLTSERSFSRKINEILLALQIERELSKNEILELYVNKIYLGNRAYGIEAAS
QVYYGKSISELSVAQLAMIAGLPKAPSAFNPVVNPARSKERRDWILGRMFRLGSLDESSY
RQALAEPETARYHGAAPELDASYIAEMARAEMVGRFGSAAYTDGFRVQTTVSSERQVAAN
NALNSGLIEYDQRHGYRGPEARLADVPRENWAQELAKYRGLSGLQPAVVTQVDTAGILAL
TRDGKEQTVSWDSMKWARPHLGINSMGPRPQRPADVVKVGDVIRIRWQDEAALFSQVPQA
QAALVSLDPQDGSIEALAGGFSFGQSNYNRAIQAKRQPGSSFKPFIYSAALDAGYTAASL
VNDSPIVFVEQGMDRIWRPKNDNNTFLGPIRMREALYKSRNLVSIRLLQTMGIDHTVDYI
SRFGFNLQDLPRNLSLALGTATLTPMEIATGWTTFANGGYKIEPYLIQRIEDREGNLVFE
ANPARVPPPDDQPAAAVADTFAAPPSRALDTDESTDAGPRYAEQVLDERTAYIMTSMLQD
VIKRGTGRRALAMGRNDIAGKTGTTNDSIDSWFSGYNADIVTTVWAGFDQPQSLGRNEYG
GTVALPIWMTFMSAVLKDMPEHPPAEPNGLLKLRIDPVSGRAATPGTPDAYFEVFKSEDS
PPPMGEVDPGYQAPGSPLPADEAAPLDLF