Protein Info for PGA1_262p00530 in Phaeobacter inhibens DSM 17395

Annotation: DNA topoisomerase 1B-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 PF21338: Top1B_N_bact" amino acids 21 to 69 (49 residues), 77.2 bits, see alignment 6.1e-26 PF01028: Topoisom_I" amino acids 83 to 246 (164 residues), 55.4 bits, see alignment E=5.9e-19

Best Hits

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 51% identity to rsk:RSKD131_2336)

Predicted SEED Role

"DNA topoisomerase IB (poxvirus type) (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7F4H0 at UniProt or InterPro

Protein Sequence (325 amino acids)

>PGA1_262p00530 DNA topoisomerase 1B-like protein (Phaeobacter inhibens DSM 17395)
MKLVYYTDDQPGITRRRRGRGFSYYMPDGSHIGDATERARLNGLGVPPAYTDVWLCPLTN
GHLQATGRDARDRKQYRYHPEWQAHRAEQKFAQLGTFGERLPVLRRWISDRLTGPVGDED
TAIAAVLALLDRGSLRIGNPSYTEENGSYGATTLRPRHVAFSGQMIQLRYTAKGGQTVEK
SLRGPALQRILERSQDLAGPELISWVDADGNAHAVRSEQVQEVLSKLCGDGVSAKTLRTW
NGSHAAFTVALDAPTLTITAMAEAAAERLHNTPTIARNSYIHPQIIALAELPDPQRAARL
SALPQRQISGLRLGEDRLIDFLNAG