Protein Info for Psest_3711 in Pseudomonas stutzeri RCH2
Annotation: Glutamate synthase domain 2
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to GLTB_ECOLI: Glutamate synthase [NADPH] large chain (gltB) from Escherichia coli (strain K12)
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 70% identity to abo:ABO_2229)MetaCyc: 61% identical to glutamate synthase subunit GltB (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- superpathway of ammonia assimilation (plants) (2/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GN67 at UniProt or InterPro
Protein Sequence (1482 amino acids)
>Psest_3711 Glutamate synthase domain 2 (Pseudomonas stutzeri RCH2) MRAGLFRPEEFKDNCGFGLIAHMQGEASHHLLKTAIQSLTCMTHRGGINADGKTGDGCGL LMQKPDAFLRAKALEHFGTELPAQYAVGMIFLDPDASKADRARARLSEEIAAQGLALVGW REVPVDTSVLGQLALERLPRIEQVFVSGEGLEEREFGIKLFFARRRAEVALADDSAFYVC SLSDKDIIYKGLMMPADLAQFYPDLGDERLATAICVFHQRFSTNTMPQWRLAQPFRFLAH NGEINTITGNRNWAQARRLKFANELLPDLESLSPLINRTGSDSSSMDNMLELLVTGGMDL FRGLRMIIPPAWQNVETMDPDLRAFYEFNSMHMEPWDGPAGVVLTDGRHAVCLLDRNGLR PARWVTTKNGYITLASEVGVWDYKPEDVLAKGRVGPGQILAVDTLTGKVLHTDDIDNHLK AQQPYKKWLRQNALRIQSTLDDNDHGSAFYDADQLKQYMKMYQVTFEERDQVLRPLAEQG QEAVGSMGDDTPMAVLSRRVRSPYDYFRQQFAQVTNPPIDPLREAIVMSLETCLGAERNV FEETAEHANRAILTTPVISPAKWRTIMNLERPGFERLVIDLNYDESIGLEAAIRNIADQA EEAVRGGKVLLVLSDRHIGPGKLPVHASLAVGAVHHRLVDTGLRCSCNILVETATARDPH HFAVLIGFGATAVYPFLAYEVLGDLIRTGEVLGDLYEVFKHYRKGISKGLMKIISKMGIS TIASYRGAQLFEAVGLSDEIVELSFRGVASRIKGARFEDLEAEQKALAAEAWSSRKPIQQ GGLLKFVYGGEYHAYNPDVVSALQMAVQSGDYSRFKEYTALVDQRPVAMLRDLLKVKLAD QPLALEEVEPLDAILKRFDSAGISLGALSPEAHEAIAAAMNRIGARSNSGEGGEDPARYG TERSSKIKQVATGRFGVTPEYLVNAEVLQIKVAQGAKPGEGGQLPGGKVNGLIARLRYAV PGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPQALVSVKLVAEPGVGTIAAGVAKAYADL ITISGYDGGTGASPLTSIRYAGSPWELGLAETHQTLRGNDLRGKVRVQTDGGLKTGLDVI KAAILGAESFGFGTAPMIALGCKYLRICHLNNCATGVATQNEQLRKDHFIGTVDMVVNFF QFVAEETREWLAKLGVRSLGELIGRTDLLELLPGETAKQNSLDLSPLLGGSNIPADKPQF CQVEKNPPFDQGLLAEEMVKLAKAAIDTKSGGEFELEICNCDRSIGARVSGEIARQHGNQ GMAGAPITFRFKGTAGQSFGVWNAGGLNLYLEGDANDYVGKGMTGGKLVVTPPQGAAYRS QDSAIIGNTCLYGATGGKLFAAGTAGERFAVRNSGAHAVVEGTGDHCCEYMTGGFVCVLG KTGHNFGSGMTGGFAYVLDMDNSFVDRVNNELVNLQRITGEAMEAYRSHLQDVLREYVAE TASEWGAELLDNLDDYLRRFWLVKPKAANLASLLSSTRANPQ