Protein Info for PGA1_262p00220 in Phaeobacter inhibens DSM 17395
Annotation: putative mandelate racemase/muconate lactonizing enzyme
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 79% identity to sit:TM1040_0846)Predicted SEED Role
"Muconolactone isomerase (EC 5.3.3.4),putative" in subsystem Catechol branch of beta-ketoadipate pathway (EC 5.3.3.4)
MetaCyc Pathways
- aromatic compounds degradation via β-ketoadipate (7/9 steps found)
- superpathway of salicylate degradation (5/7 steps found)
- catechol degradation III (ortho-cleavage pathway) (4/6 steps found)
- catechol degradation to β-ketoadipate (2/4 steps found)
- 4-methylcatechol degradation (ortho cleavage) (4/7 steps found)
- mandelate degradation to acetyl-CoA (7/18 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (14/35 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.3.3.4
Use Curated BLAST to search for 5.3.3.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7E661 at UniProt or InterPro
Protein Sequence (391 amino acids)
>PGA1_262p00220 putative mandelate racemase/muconate lactonizing enzyme (Phaeobacter inhibens DSM 17395) MTTPYSASGAVPVGAGRGKEDSGPRIRRIETFCSPLVGFVRVTSEDGRQGWGQVSTYNSD LTCEILHRQVAPWALGRGMDAMEAVIAEIPLREHKYPGTYLRRAMAGLDTAVWDWRGRVA EKPVAELLGGSSGPIRAYASSMRRDITPKDEAERLKALRDDLGFDAFKVRVGAECGEDRD EWPGRTEAIIPQIRTALGEGVALLVDGNSGFSPKRAIEVGQLLQDHGYEHFEEPCPYWML EQTAEVTRALDLDVAGGEQDWDLQTWHRMIEMRAVDIIQPDILYLGGMTRSMEIARLGAA AGLPCTPHCANLSMVTLFTMHMMRAVDLPGRYLEFSIEGEDYYPWQRDLFVSDPFVICDG QATVSEVPGWGVEINPEWLAKSKYTCSEDTP