Protein Info for HP15_3546 in Marinobacter adhaerens HP15

Annotation: copper-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 873 transmembrane" amino acids 205 to 226 (22 residues), see Phobius details amino acids 238 to 261 (24 residues), see Phobius details amino acids 274 to 297 (24 residues), see Phobius details amino acids 303 to 322 (20 residues), see Phobius details amino acids 458 to 478 (21 residues), see Phobius details amino acids 484 to 506 (23 residues), see Phobius details amino acids 799 to 818 (20 residues), see Phobius details amino acids 824 to 845 (22 residues), see Phobius details PF00403: HMA" amino acids 10 to 50 (41 residues), 27.7 bits, see alignment 5.8e-10 amino acids 124 to 179 (56 residues), 53 bits, see alignment 7.4e-18 TIGR01511: copper-translocating P-type ATPase" amino acids 254 to 849 (596 residues), 617.3 bits, see alignment E=6e-189 TIGR01525: heavy metal translocating P-type ATPase" amino acids 272 to 848 (577 residues), 627.1 bits, see alignment E=6.9e-192 TIGR01494: HAD ATPase, P-type, family IC" amino acids 311 to 818 (508 residues), 293.5 bits, see alignment E=4.7e-91 PF00122: E1-E2_ATPase" amino acids 340 to 520 (181 residues), 201.1 bits, see alignment E=2.3e-63 PF00702: Hydrolase" amino acids 538 to 753 (216 residues), 130.6 bits, see alignment E=1.9e-41

Best Hits

Swiss-Prot: 47% identical to COPA_VIBCH: Copper-exporting P-type ATPase (copA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 83% identity to maq:Maqu_3789)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PG46 at UniProt or InterPro

Protein Sequence (873 amino acids)

>HP15_3546 copper-translocating P-type ATPase (Marinobacter adhaerens HP15)
MTDTPVLQTTLSISGASCQGCAKKIRNALEPLTGDTGLVDVDLEKQTVALPEGVDASEAA
RIVTETGYPAEPFEEDAKPGSCCSPAHQDKGHSNDSAEDRATGERSAPASSDATTGADEQ
IHLSVTGATCASCVNTIEKALMSVSGISHAHMNLADNTATATGDADPESLVKAVESAGYG
ASVIADEDEADDRKQEEDRKQYKTLLVKMAVSLGLGLGLMVWGMGFGTMMVTDANQGTWL
GLGLLTLTVMAATGGHFYTGAWKAFRHHNANMDTLIALGTGTAWLYSIVVASIPGALPEM
ARHVYFEASAMIIGLINLGQALELRAKGKTSEAVRRLLDLRAKTARVIRDGEEQDLPVEE
VRKGDHIRVRPGEKLPVDGVIAEGSTRMDESMLTGEPMPVSKSEGDEVSAGTLNTHGSIV
YEATRVGSETALAQIIKLVKKAQGSKPAIGRLADKISSVFVPTVMLIAVVAALVWYNVGP
EPAVVHMMVAATTVLIIACPCALGLATPMSVMVGVGKAAEYGALIRQGDALQTAGKLDLV
ILDKTGTITEGHPAVTRVHAIDGDEQRLLVLAAGLEQHSEHPLAEAVLEKAKAQSVQPAK
VTGFEALNGKGVQGKLDGEPLRLGNRRWLEDQGIGLDGLAEAARAITEEAGTPLFLALGN
EALGVIGVADAIKPDSKAAIRRLHEAGIKVMMVTGDVDATARAIAKKAGIDDYRAEVLPE
DKAEVVSEMRGKGHVVAMVGDGINDAPALAAADVGFAIGTGTDVAIESAGITLMRGSLHG
VPDAIEISRATVKNIHQNLFGAFVYNTMGIPVAAGLLYPVWGILMSPILAGAAMSLSSVT
VVSNANRLRLFRTSHRPERSDSKSDPKEQQERN