Protein Info for Psest_3666 in Pseudomonas stutzeri RCH2

Annotation: Parvulin-like peptidyl-prolyl isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF09312: SurA_N" amino acids 37 to 154 (118 residues), 139.8 bits, see alignment E=1.4e-44 PF13623: SurA_N_2" amino acids 39 to 117 (79 residues), 33.4 bits, see alignment E=1.2e-11 PF13624: SurA_N_3" amino acids 39 to 153 (115 residues), 49.3 bits, see alignment E=1.6e-16 PF13616: Rotamase_3" amino acids 181 to 285 (105 residues), 49.6 bits, see alignment E=1.7e-16 amino acids 285 to 393 (109 residues), 96.5 bits, see alignment E=4.9e-31 PF00639: Rotamase" amino acids 191 to 282 (92 residues), 66 bits, see alignment E=1.6e-21 amino acids 300 to 390 (91 residues), 80.9 bits, see alignment E=3.4e-26 PF13145: Rotamase_2" amino acids 192 to 296 (105 residues), 32.7 bits, see alignment E=3.5e-11 amino acids 312 to 395 (84 residues), 29.3 bits, see alignment E=4e-10

Best Hits

Swiss-Prot: 76% identical to SURA_PSEF5: Chaperone SurA (surA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03771, peptidyl-prolyl cis-trans isomerase SurA [EC: 5.2.1.8] (inferred from 95% identity to psa:PST_0728)

MetaCyc: 38% identical to chaperone SurA (Escherichia coli K-12 substr. MG1655)
Peptidylprolyl isomerase. [EC: 5.2.1.8]

Predicted SEED Role

"Survival protein SurA precursor (Peptidyl-prolyl cis-trans isomerase SurA) (EC 5.2.1.8)" in subsystem Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GS61 at UniProt or InterPro

Protein Sequence (437 amino acids)

>Psest_3666 Parvulin-like peptidyl-prolyl isomerase (Pseudomonas stutzeri RCH2)
MNVKIKLSEVLRPLALGALLLSGATLAPAALAQVKPLDRVVAIVDNDVIMQSQLQQRIRE
VQQTIEKRGADAPPMDVLQQQVLERLITENLQLQIGERSGIRIADEELNQAMGTIAQRNN
MSLEQFREALSRDGLSLESAREQIRREMVISRVRQRRVAERIQVSNQEVQNFLASDLGKL
QLSEEYHLANILIPVPEAADSATIQAAERTAMDTYQQLQQGADFARLAVSRSGSENALEG
GDMGWRKAAQLPPPFDAVVRELSIGEVTEPVRTPPGFIMLKLLEKRGGETQVRDEVHVRH
ILIKPSAIRSEEEARLLVQRLRDRISAGEDFAQLAKSFSEDPGSALNGGDLNWIDPASLV
PEFREVMANTASGELSPVFKSPYGWHILEVLGRRATDASEQFREQQALSLLRNRKYDEEL
QAWLRQIRDEAYVEIKL