Protein Info for Psest_3654 in Pseudomonas stutzeri RCH2

Annotation: succinate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 21 to 482 (462 residues), 565.7 bits, E=3.4e-174
• TIGR01780: succinate-semialdehyde dehydrogenase
  • amino acids 33 to 481 (449 residues), 738.7 bits, E=1.2e-226

Best Hits

Swiss-Prot: 74% identical to DAVD_PSEAE: Glutarate-semialdehyde dehydrogenase (davD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 98% identity to psa:PST_0740)

MetaCyc: 74% identical to glutarate semialdehyde dehydrogenase (Pseudomonas aeruginosa)
Glutarate-semialdehyde dehydrogenase. [EC: 1.2.1.20]

Predicted SEED Role

"Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)" (EC 1.2.1.16, EC 1.2.1.20)

MetaCyc Pathways

• L-lysine degradation X (5/6 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• L-lysine degradation IV (3/5 steps found)
• L-lysine degradation I (4/7 steps found)
• L-lysine degradation III (2/6 steps found)
• superpathway of L-lysine degradation (11/43 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Lysine degradation
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.20

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.20

Sequence Analysis Tools

See L0GQR9 at UniProt or InterPro

Protein Sequence (488 amino acids)

>Psest_3654 succinate-semialdehyde dehydrogenase (Pseudomonas stutzeri RCH2)
MTLQLGQPDLLRQTAYLNGEWCEADSGARTEIFNPATGELIGAVPNMGRGETRRAIEAAQ
AAQPAWRALTAKERAARLRRWYELMLENQEDLARIMTAEQGKPLAEARGEVAYAASFLEW
FAEEGKRLYGDVIPAHAGDKRILVQKEPVGVTAAITPWNFPSAMITRKAGPALAAGCAMV
LKPAPQTPFSALALAALAERAGIPAGLLSVITADAATSREVGAELCENPIVRKLSFTGST
AVGIKLMQQCAPTLKKLSLELGGNAPFIVFDDADLDAAVEGAMISKYRNAGQTCVCANRI
YVQDGIYDAFVDKLSAAVARLKVGNGAEEGVTTGPLIDAAAVAKVQRHLQDALDKGATLL
AGGKPHALGGNFFEPTLVGGVTSEMAVAREETFGPLAPLFRFRDEDEVIRQANDTEFGLA
AYFYARDLSRVFRVAEALEYGMVGINTGVISTEVAPFGGMKASGLGREGSKYGLDEYVEI
KYLCLGGI