Protein Info for PGA1_c36350 in Phaeobacter inhibens DSM 17395

Updated annotation (from data): Imidazolonepropionase (EC 3.5.2.7)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (IMIDAZOLONEPROPIONASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: imidazolonepropionase HutI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01224: imidazolonepropionase
  • amino acids 29 to 403 (375 residues), 454.2 bits, E=1.5e-140
• PF01979: Amidohydro_1
  • amino acids 64 to 382 (319 residues), 73.7 bits, E=1.7e-24
• PF07969: Amidohydro_3
  • amino acids 199 to 380 (182 residues), 61 bits, E=1.6e-20

Best Hits

Swiss-Prot: 75% identical to HUTI_RUEST: Imidazolonepropionase (hutI) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K01468, imidazolonepropionase [EC: 3.5.2.7] (inferred from 75% identity to sit:TM1040_3004)

MetaCyc: 60% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Sequence Analysis Tools

See I7DW49 at UniProt or InterPro

Protein Sequence (404 amino acids)

>PGA1_c36350 Imidazolonepropionase (EC 3.5.2.7) (Phaeobacter inhibens DSM 17395)
MGDTDSYLICDATLATMTGSDPAYGLVPDGLIVVQNGWIRWCGSEAQLPKDYSDWPRVSM
GGRLVTPGLIDCHTHIVFGGNRAMEFEMRLNGASYEDVARAGGGIVSTVSATRTASLEDL
VQGALPRLDALIAEGATVVEVKSGYGLDRETELNMLRAARRLAVLRSVTVNTTFLGAHAT
PGEYKGRDDAYIDEVCIPALRAAHAEGLVDAVDGFCENIAFAPAQIERVFKAARELGLPV
KLHAEQLSHQGGTALSAQYGALSVDHVEYATEDDAKMMAAAGSVAVILPGAFYTIREIQA
PPIEHFRSHGVPMALATDCNPGSSPLTSLLLTMNMGCTLFRMTPEEALAGVTRNAASALG
LTDRGQISAGMRADLAVWDVETPGELAYRIGFNPLYTRIYEGNQ