Protein Info for HP15_3486 in Marinobacter adhaerens HP15
Annotation: 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 36% identical to YKWC_BACSU: Uncharacterized oxidoreductase YkwC (ykwC) from Bacillus subtilis (strain 168)
KEGG orthology group: K00042, 2-hydroxy-3-oxopropionate reductase [EC: 1.1.1.60] (inferred from 87% identity to maq:Maqu_3742)Predicted SEED Role
"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)
MetaCyc Pathways
- glycolate and glyoxylate degradation I (2/4 steps found)
- superpathway of glycol metabolism and degradation (4/7 steps found)
- D-glucarate degradation I (1/4 steps found)
- D-galactarate degradation I (1/4 steps found)
- superpathway of D-glucarate and D-galactarate degradation (1/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (5/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.60
Use Curated BLAST to search for 1.1.1.60
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E4PFY6 at UniProt or InterPro
Protein Sequence (303 amino acids)
>HP15_3486 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenase (Marinobacter adhaerens HP15) MTSTKPHIAFLGIGLMGTPMTRNLLNAGFPMTLWNRTSSKCEPFASEAVIAESPAEAVAN ADVVITMLENSDVVEQVLVAQGAIDALKPGALVIDMSSIQPSVARRHGELVAEQGAGYVD APVSGGTVGAAEARLSIMAGGSEADVDRARPVFEALGKCTRIGPVGAGQLAKLANQAIVG ITIGAVSEALLLAAKGGADPAAVREALLGGFAGSRILELHGQRMIDREFAPGAPARIQLK DMRMILDEARAEGLTLPLAQQTHNEYLSLVANGHSDVDHSGLLLELEHLNGALLGSLGRG PKE