Protein Info for Psest_3576 in Pseudomonas stutzeri RCH2

Annotation: excinuclease ABC, A subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 947 TIGR00630: excinuclease ABC subunit A" amino acids 3 to 925 (923 residues), 1488.1 bits, see alignment E=0 PF17760: UvrA_inter" amino acids 130 to 237 (108 residues), 135.8 bits, see alignment E=1.9e-43 PF17755: UvrA_DNA-bind" amino acids 291 to 399 (109 residues), 115 bits, see alignment E=7.4e-37

Best Hits

Swiss-Prot: 76% identical to UVRA_ECOL6: UvrABC system protein A (uvrA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03701, excinuclease ABC subunit A (inferred from 76% identity to abo:ABO_0427)

MetaCyc: 76% identical to UvrABC excision nuclease subunit A (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit A" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRR3 at UniProt or InterPro

Protein Sequence (947 amino acids)

>Psest_3576 excinuclease ABC, A subunit (Pseudomonas stutzeri RCH2)
MDKILIRGARTHNLKNIDLTLPRDKLIVITGLSGSGKSSLAFDTLYAEGQRRYVESLSAY
ARQFLSMMEKPDVDTIEGLSPAISIEQKSTSHNPRSTVGTITEIYDYLRLLYARVGTPRC
PDHDAPLEAQTVSQMVDQVLALPEGRKLMLLAPVIRERKGEHLAVFDELRAQGFVRARVN
GRLYELDELPKLDKQKKHSIDVVVDRFKVRGDLQQRLAESFETALKLADGIALVASMEEE
DESEEMIFSARFACPICGHSISELEPKLFSFNNPAGACPTCDGLGVKQFFDAKRLVNGEL
TLAEGAIRGWDRRNVYYFQMLGSLASHYGFSLDEPFDSLAADHQKSILRGSGRESVEFRY
LNDRGDIVKRSHPFEGIIPNLERRYRETESNSVREELAKYLSTQPCPDCRGTRLRREARH
VWVGDRTLPAVTAMPIGDATDYFGGLSLSGRRGEIADKILKEIRERLQFLVNVGLDYLTL
DRSADTLSGGEAQRIRLASQIGAGLVGVMYILDEPSIGLHQRDNERLLGTLRHLRDIGNT
VIVVEHDEDAIRLADYVVDIGPGAGVHGGRIVAEGTPDEVMAHPDSLTGKYLSGRVKINY
RPERTRRDPKKLLKLKGARGNNLRNVDLEIPVGLLTCITGVSGSGKSTLINNTLFPITAT
ALNGASTLEVAPHDSFDGLQHLDKVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAGVPE
ARSRGYGPGRFSFNVKGGRCEACQGDGVIKVEMHFLPDIYVPCDVCKGKRYNRETLEVKY
KGKSITEVLDMTIEEAREFFDPVPAVARKLQTLMDVGLSYIKLGQSATTLSGGEAQRVKL
SRELSKRDTGKTLYILDEPTTGLHFADIQQLLDVLHRLRDHGNTVVVIEHNLDVIKTADW
LVDLGPEGGSKGGQIIACGTPEEVAGMAQSHTGHFLKPLLERDRSAG