Protein Info for Psest_3550 in Pseudomonas stutzeri RCH2

Annotation: 3,4-dihydroxy-2-butanone 4-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 4 to 199 (196 residues), 240.5 bits, E=6.3e-76
• PF00926: DHBP_synthase
  • amino acids 7 to 198 (192 residues), 284.7 bits, E=2.9e-89
• PF00925: GTP_cyclohydro2
  • amino acids 207 to 357 (151 residues), 116.2 bits, E=1.2e-37

Best Hits

Swiss-Prot: 79% identical to RIBB_PSEAE: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 99% identity to psa:PST_0837)

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

See L0GRV7 at UniProt or InterPro

Protein Sequence (359 amino acids)

>Psest_3550 3,4-dihydroxy-2-butanone 4-phosphate synthase (Pseudomonas stutzeri RCH2)
MALNTAEELIEDIRAGKMVILMDDEDRENEGDIIVASECVTAEHINFMARFARGLICMPM
TRERCELLKLPLMAPRNGSGFGTKFTVSIEAAEGVTTGISAADRARTVQAAVARAAKAED
IVSPGHIFPLMAQPGGVLARAGHTEAACDLARMAGFEASGVICEIMNDDGTMARRPELEL
FAEQHGLKIGTIADLIHYRMIHERTVERVSEQPLETELGQFNLVTYRDGVEDTVHMALTR
GEISPEEPTLVRVHNMEPLRDLLLVTVPGRWSLRAAMSEVAKAGKGVVLLLGNPLTGPQL
LAQLTRQQPPTPATYSTVGAGSQILRDLGVRKMRLMSAPMKFNAISGFDLEVVEYLPAE