Protein Info for Psest_3524 in Pseudomonas stutzeri RCH2

Annotation: ribose-phosphate pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 TIGR01251: ribose-phosphate diphosphokinase" amino acids 11 to 294 (284 residues), 168.7 bits, see alignment E=7.3e-54 PF13793: Pribosyltran_N" amino acids 11 to 118 (108 residues), 46.7 bits, see alignment E=2.7e-16 PF00156: Pribosyltran" amino acids 155 to 251 (97 residues), 37.8 bits, see alignment E=1.2e-13

Best Hits

Swiss-Prot: 36% identical to KPRS_METKA: Ribose-phosphate pyrophosphokinase (prs) from Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

KEGG orthology group: K00948, ribose-phosphate pyrophosphokinase [EC: 2.7.6.1] (inferred from 93% identity to psa:PST_0859)

Predicted SEED Role

"Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)" in subsystem De Novo Purine Biosynthesis or Pentose phosphate pathway (EC 2.7.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.1

Use Curated BLAST to search for 2.7.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMR0 at UniProt or InterPro

Protein Sequence (298 amino acids)

>Psest_3524 ribose-phosphate pyrophosphokinase (Pseudomonas stutzeri RCH2)
MQSQLLYFDDERAAALRLAEACGLTAAQIERHRFPDDELRLRLPLADGEAIAEQLVIYRG
LDHPNEKLVELLLIAGEARRLGAKRLVLVTPYLAYMRQDIAFNPGEVVSQRVIGQLLAGQ
FDGLITVDPHLHRVATLQEAVPLEDAVTLCAAPALARLIAERHPDALLIGPDAEALQWIE
AAAAEHGFAHGVCSKVRHGDHQVEIALPELQFAGRHVVLLDDVASSGRTLAQAAEKLLAA
GAASVDVAVTHALFAGDALQVIRDAGVGHVWSTDCIAHGSNAVSMAPQLAEALRPLLR