Protein Info for Psest_3513 in Pseudomonas stutzeri RCH2

Updated annotation (from data): Homogentisate 1,2-dioxygenase (EC 1.13.11.5)
Rationale: Specifically important for utilizing L-tyrosine disodium salt. Automated validation from mutant phenotype: the predicted function (HOMOGENTISATE-12-DIOXYGENASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Homogentisate 1,2-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 PF20510: HgmA_N" amino acids 86 to 232 (147 residues), 60.9 bits, see alignment E=1.2e-20 PF04209: HgmA_C" amino acids 261 to 373 (113 residues), 23.8 bits, see alignment E=3.5e-09

Best Hits

Swiss-Prot: 66% identical to Y1345_VIBCH: Putative dioxygenase VC_1345 (VC_1345) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00451, homogentisate 1,2-dioxygenase [EC: 1.13.11.5] (inferred from 99% identity to psa:PST_0870)

Predicted SEED Role

"Homogentisate 1,2-dioxygenase (EC 1.13.11.5)" in subsystem Homogentisate pathway of aromatic compound degradation (EC 1.13.11.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQE9 at UniProt or InterPro

Protein Sequence (379 amino acids)

>Psest_3513 Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (Pseudomonas stutzeri RCH2)
MSRKWISFPIREGESSRQAHCDFPQGTYEREMGREGFFGPASHLHHKHPPTGWIDWEGPL
RPHAFNFNQIPSEGDCPLQAPLALHNADVKLRLWKTNGAMRHLVRNGDGDELLFIHEGAG
HLYCDFGHLEFRDGDYLMIPRGTAWRIEATQPVFMLLIENTDGAYQLPDKGLVGPHAIFD
AAVLDHPRLDDAFRAQQDENPWQIRIKRRDQITTVTYPYNPLDVVGWHGDNTVVRLNWRD
IRPLMSHRYHLPPSAHTTFVANGFVVCTFTPRPVESDPGALKVPFFHNNDDYDEVLFYHR
GNFFSRDNIEQGMVTFHPCGFPHGPHPKALKKAQDDPATFADEVAVMIDTRRALEVGEAA
AAVDVPEYVNSWRAPGKES