Protein Info for Psest_3486 in Pseudomonas stutzeri RCH2

Annotation: respiratory nitrate reductase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1251 TIGR01580: nitrate reductase, alpha subunit" amino acids 2 to 1236 (1235 residues), 2475.7 bits, see alignment E=0 PF14710: Nitr_red_alph_N" amino acids 3 to 40 (38 residues), 60.3 bits, see alignment (E = 2.7e-20) PF00384: Molybdopterin" amino acids 108 to 832 (725 residues), 230.1 bits, see alignment E=6.1e-72 PF01568: Molydop_binding" amino acids 1088 to 1203 (116 residues), 74.9 bits, see alignment E=7.5e-25

Best Hits

Swiss-Prot: 73% identical to NARG_ECOLI: Respiratory nitrate reductase 1 alpha chain (narG) from Escherichia coli (strain K12)

KEGG orthology group: K00370, nitrate reductase 1, alpha subunit [EC: 1.7.99.4] (inferred from 69% identity to aeh:Mlg_1003)

MetaCyc: 71% identical to nitrate reductase Z subunit alpha (Escherichia coli K-12 substr. MG1655)
1.97.1.-; RXN0-3501 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase alpha chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRJ4 at UniProt or InterPro

Protein Sequence (1251 amino acids)

>Psest_3486 respiratory nitrate reductase, alpha subunit (Pseudomonas stutzeri RCH2)
MSHLLDQLRFFNRKQGEFADGHGETRIESRDWENVYRSRWQYDKIVRSTHGVNCTGSCSW
KIYVKNGLITWETQQTDYPRTRNDLPNHEPRGCPRGASYSWYIYSANRLKYPKVRKPLLK
LWREARRNMSPVDAWASIVEDKAKAESYKSKRGMGGFIRSSWEEVNEIIAAANVYTVKQY
GPDRVVGFSPIPAMSMVSYAAGSRYLSLIGGVCLSFYDWYCDLPPASPQVWGEQTDVPES
ADWYNSNYIIAWGSNVPQTRTPDAHFFTEVRYKGTKTVAITPDYAEVAKLTDLWLNPKQG
TDAALAQAFAHVIFKEFHLEKPSAYFRDYAKRYTDLPVLVRLNEKDGSYIADRFLRASDL
ADNLGQENNPEWKTIAVDGTTGELVSPLGSIGYRWGEKGKWNIEAREGKDGRDVDLSLTQ
IDGGETAEVAFPYFGGILHEHFQHAEGESIQLRRVPVRSITLADGSTTKVATVFDLMAAN
LGIDRGLGGANVASSYDDASVPGTPAWQEVITGVSREKAIQIAREFADNADKTHGRSMII
VGAAMNHWYHMDMNYRGLINMLMLCGCVGQTGGGWAHYVGQEKLRPQCGWLPLAFGLDWH
RPPRQMNGTSFFYNHSSQWRHEKMSIHEVLSPLADKSQFPEHMLDYNIRAERAGWLPSAP
QLNRNPLQICREAEAAGMSPVDYVTQSLKDGSLKFACEQPDNPDNFPRNMFIWRSNLLGS
SGKGHEYMLKYLLGTKNGVMNEDLGKRDGFKPLEAEWQEDGAIGKLDLVTTLDFRMSSTC
VYSDIVLPTATWYEKDDMNTSDMHPFIHPLSAAIDPAWEARSDWEIYKGIAKSFSKMAEG
HLGVEKDLVTVPLLHDSPGELAQPFGGTDWKTAGVDPQPGKNCPNMTVVERDYPATYKKF
SSLGPLLDKLGNGGKGINWNTQDEVDFLGELNYKVRDEGVSQGRPQIESAIDAAEVILSL
APETNGHVAVKAWAALSEFTGRDHSHLALPKEHEAIRFRDIQAQPRKIISSPTWSGLEDE
HVSYNAGYTNVHEYIPWRTITGRQQFFQDHPWMQAFGEQLMSYRPPINTRTIDYVKGKKS
NGNTEIVLNWITPHQKWGIHSTYSDNLIMLTLSRGGPIVWMSEVDAKKAGIEDNDWIECF
NANGALTARAVVSQRVMEGMVMMYHAQERIVNVPGSETTKTRGGHHNSVTRVVLKPTHMI
GGYAQQAYGFNYYGTVGCNRDEFVVVRKMAKVDWLDGPNGNDLPQPLPQDI