Protein Info for Psest_3476 in Pseudomonas stutzeri RCH2

Annotation: Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 PF00072: Response_reg" amino acids 3 to 112 (110 residues), 100.9 bits, see alignment E=5e-33 PF00486: Trans_reg_C" amino acids 145 to 215 (71 residues), 73.1 bits, see alignment E=1.6e-24

Best Hits

Swiss-Prot: 48% identical to QSEB_ECOLI: Transcriptional regulatory protein QseB (qseB) from Escherichia coli (strain K12)

KEGG orthology group: K02483, two-component system, OmpR family, response regulator (inferred from 97% identity to psa:PST_0906)

Predicted SEED Role

"Two-component system response regulator QseB" in subsystem Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRH6 at UniProt or InterPro

Protein Sequence (219 amino acids)

>Psest_3476 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain (Pseudomonas stutzeri RCH2)
MRILLVEDDRALGEGIRTALKPEGYTVDWLQDGASALHALSHESFELAILDLGLPRLDGL
EVLKRLRAAANPVPVLVLTARDATGDRIAGLDAGADDYLVKPFDVAELKARLRALLRRSF
NRPEPSLEYRGILLDPVNQQVSYQGTPINLPRKEFVLLHELLAQPGRVLTRDRLQQVLYG
WDEEVESNALEVHIHHLRKKFFPELIRTVRGVGYLVDKC