Protein Info for PGA1_c34530 in Phaeobacter inhibens DSM 17395

Annotation: chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 TIGR02350: chaperone protein DnaK" amino acids 3 to 601 (599 residues), 964 bits, see alignment E=1.4e-294 PF00012: HSP70" amino acids 4 to 601 (598 residues), 913.3 bits, see alignment E=6e-279 PF06723: MreB_Mbl" amino acids 134 to 373 (240 residues), 54.6 bits, see alignment E=8.2e-19

Best Hits

Swiss-Prot: 95% identical to DNAK_RUEST: Chaperone protein DnaK (dnaK) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 95% identity to sit:TM1040_0010)

MetaCyc: 66% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E5H7 at UniProt or InterPro

Protein Sequence (639 amino acids)

>PGA1_c34530 chaperone protein DnaK (Phaeobacter inhibens DSM 17395)
MSKVIGIDLGTTNSCVAIMDGSQPRVIENAEGARTTPSIVAFTDDERLVGQPAKRQAVTN
PDNTVFGVKRLIGRRFDDSDLAKDKKNLPFAVINGGNGDAWVEAKGEKYSPSQISAFILG
KMKETAESYLGEEVTQAVITVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGL
DKEETQTIAVYDLGGGTFDVTILEIDDGLFEVKSTNGDTFLGGEDFDMRIVNYLADQFKK
EHGVDLTKDKMALQRLKEAAEKAKIELSSSSQTEINQPFISMDPSSGQPLHMVMKLTRAK
LESLVGDLIKASMKPCAAALKDAGLSASDVDEVVLVGGMTRMPKVIEEVSKFFGKEPHKG
VNPDEVVAMGAAIQAGVLQGDVKDVVLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQ
VFSTAEDNQNAVTIRVFQGEREMAADNKMLGQFNLEDIPPAPRGMPQIEVTFDIDANGIV
SVGAKDKATGKEQNITIQASGGLSDDDIEKMVKDAEDNAEADKERRELIEAKNQAESLIH
STEKSMEEHGDKVDPTTIEAIELAIAALKDDLENEKSTAEKIKSGVQNVTEAAMKLGEAI
YKSSQEEAADEPTAADDAAGPGDDDIVDAEFEDLDDNKR