Protein Info for PGA1_c34420 in Phaeobacter inhibens DSM 17395

Annotation: putrescine-binding periplasmic protein PotF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF01547: SBP_bac_1" amino acids 32 to 296 (265 residues), 49.8 bits, see alignment E=1e-16 PF13416: SBP_bac_8" amino acids 37 to 325 (289 residues), 92.4 bits, see alignment E=8.6e-30 PF13343: SBP_bac_6" amino acids 72 to 310 (239 residues), 50.9 bits, see alignment E=3.1e-17

Best Hits

Swiss-Prot: 49% identical to POTF_ECOLI: Putrescine-binding periplasmic protein (potF) from Escherichia coli (strain K12)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 78% identity to sil:SPO3469)

MetaCyc: 49% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7ERU2 at UniProt or InterPro

Protein Sequence (361 amino acids)

>PGA1_c34420 putrescine-binding periplasmic protein PotF (Phaeobacter inhibens DSM 17395)
MTLKTMTLTAIVALSSAAAVAEEVRVYNWSDYIDEDLLEKFETETGIDLIYDVFDSNELL
ETKMLAGGSGYDVVVPTGSFLARQIQAGAFQKLDTSKLSNAGNMWDVIEDRTARYDPDNL
YSVNYMWGTTGIGANTAKVEEALGADAPIDSLELVFNPDNIEKLANCGVYFLDAPDEMIP
AALKYIGEDPNSMDPDVVAKAEPVLMAIRPYVKKFHSSEYINALANGDICVAFGWSGDIL
QARDRADEADNGVEIVFNAPKEGALMWFDQMAIPVDAPNPDGAHKFLNFIMEAENMAAAS
NYVYYANGNKASQEFLEEDVIGDPAIYPSEETLKNLYIKEAYPPKVQRKATRMWTKIKSG
T