Protein Info for Psest_3444 in Pseudomonas stutzeri RCH2

Annotation: Aerobic-type carbon monoxide dehydrogenase, large subunit CoxL/CutL homologs

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01315: Ald_Xan_dh_C
  • amino acids 22 to 135 (114 residues), 82.1 bits, E=5.3e-27
• PF02738: MoCoBD_1
  • amino acids 158 to 381 (224 residues), 156 bits, E=1.4e-49
• PF20256: MoCoBD_2
  • amino acids 404 to 676 (273 residues), 188.5 bits, E=2.9e-59

Best Hits

KEGG orthology group: K11177, xanthine dehydrogenase YagR molybdenum-binding subunit [EC: 1.17.1.4] (inferred from 94% identity to psa:PST_0926)

Predicted SEED Role

"Xanthine dehydrogenase, molybdenum binding subunit (EC 1.17.1.4)" in subsystem Purine Utilization (EC 1.17.1.4)

MetaCyc Pathways

• adenosine nucleotides degradation II (4/5 steps found)
• guanosine nucleotides degradation II (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• ureide biosynthesis (5/7 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• superpathway of purines degradation in plants (11/18 steps found)
• caffeine degradation III (bacteria, via demethylation) (2/7 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (2/10 steps found)
• theophylline degradation (1/9 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Sequence Analysis Tools

See L0GME5 at UniProt or InterPro

Protein Sequence (733 amino acids)

>Psest_3444 Aerobic-type carbon monoxide dehydrogenase, large subunit CoxL/CutL homologs (Pseudomonas stutzeri RCH2)
MNSANSPLGKPLDRVDGPLKVTGKACYAAEAEVPGLLYGAVVSSSIARGRIKGIDAAAAE
ALPGVRLVLTHQNRPPVASYDEPYEDDDAADGSPFRPLYNDRVLYSGQPLALVVAETQAL
ARHGASLVHVEYEVEAHQTDLQAAREQAHEAPAELPEPRGDFDSAFAAAAKRIDCEYGTP
VEHHNPMEPHTSIVQYQPGGELLIHDKTQGVQNCQRYLEQVFDMQGKIRVLAPFVGGAFG
SGLRPQYQLPLAVMAALKLKASVRVELTRQQMFTFGYRPRTFQQLKLAADGEGRLRAIEH
KAIGQTSRFEDFTEHEVEWSGMLYACDNVRLGYRLAPLDVYTPLDMRAPGATIGVYALEC
AMDELAHEVGIDPLELRLRNYTDINGNEGKRYSSKELRACYQQGAERFGWSRRPAQPRSL
RDGHQLVGMGMATGVWEAMQMPASARACIDADGKLLVTSATADIGTGTYTAMTQIAADAM
GLSMAEVEFRLGDSSLPQAPLEGGSATVSSVGSAVQRACAGLRQKLLDAVQQSPASPFTG
ANLETVEFVDGQLRLKTGEHAVALRDIVQVSGALEAEASVKPDEKRDAWATGTHSAVFVE
VRVDEDLGTIKVSRVVSAIAAGRIVNPKTAGNQIVGGVVWGIGQALHEETLIDHRLGRYM
NHNLAEYHIPVNADIPEIDVIFVEEHDEVVNDLGSKGVGEIGIVGAAAAVANAIYNATGK
RVRDLPITLDKLL