Protein Info for Psest_3405 in Pseudomonas stutzeri RCH2

Annotation: Type I restriction-modification system methyltransferase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 658 PF12161: HsdM_N" amino acids 10 to 140 (131 residues), 101.9 bits, see alignment E=5e-33 PF02384: N6_Mtase" amino acids 155 to 452 (298 residues), 197.4 bits, see alignment E=3.8e-62

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 93% identity to psa:PST_0647)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR81 at UniProt or InterPro

Protein Sequence (658 amino acids)

>Psest_3405 Type I restriction-modification system methyltransferase subunit (Pseudomonas stutzeri RCH2)
MNTENHSQTAAFLWSIADLLRGDFKQSQYGRIILPFTLLRRMECVLAPTKDEVIKQTFAQ
EGRPDTVREMILLRAAGQQFFNASPLTLGTLSDTQTAADLMSYVQAFSKDAREIFEHFHF
EDFVQQLETANLLYQVVQRFAATDLSPEFISNFGMGIIFEELIRKFAESSNETAGEHFTP
RDIVHLTTSLVITNQDHKLTPNSIVTIYDPTAGTGGFLSEGDEYIQSISEKVSVSLHGQE
LNPESYAICKADMLIKGQDVASIKLGNTLSNDQLADKRFDFMLSNPPFGVEWKKVQKQIT
DEHSHKGFDGRFGPGLPRVSDGSLLFLLHLVSKMRDPRDGGSRIGIILNGSPLFTGGAGS
GESEIRRYLLQNDLVEAIVALPTDMFYNTGIATYVWILSNHKAAARQGKVQLIDGSQHYA
KMRKSLGSKRQYITEDQISELVRLYGSFEQTAQSKIFPIEAFGYRRITVERPLRLNFQTS
PERIGKVLEEKAIVKLGSDGWQPIQRALKSMDASVLHRNREQFSKLLKKALTAHSVSLST
PELKALMSALSERDPEADICMTKGQPEADAGLRDNENVPLGESVYDYFQREVIPHVPDAW
IDESKTDAQDGEVGIVGFEIPFNRHFYVFQPPRPLAEIDSDLKACTDRIKQMIEGLSA