Protein Info for PGA1_c03450 in Phaeobacter inhibens DSM 17395
Annotation: argininosuccinate lyase ArgH
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 90% identical to ARLY_RUEPO: Argininosuccinate lyase (argH) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 94% identity to sit:TM1040_3732)MetaCyc: 43% identical to argininosuccinate lyase (Homo sapiens)
Argininosuccinate lyase. [EC: 4.3.2.1]
Predicted SEED Role
"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)
MetaCyc Pathways
- superpathway of arginine and polyamine biosynthesis (17/17 steps found)
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- urea cycle (4/5 steps found)
- nitric oxide biosynthesis II (mammals) (2/3 steps found)
- canavanine biosynthesis (2/4 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.3.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7DX95 at UniProt or InterPro
Protein Sequence (456 amino acids)
>PGA1_c03450 argininosuccinate lyase ArgH (Phaeobacter inhibens DSM 17395) MWGGRFAAGPDAIMEAINASIGFDKRMAAQDIAGSRAHAAMLAATGVITDNDAEAIREGL LTVLSEIEGGTFQFSTALEDIHMNVEARLKDIIGEPAGRLHTGRSRNDQVATDFKLWVRD QLDAAESGLLALIRALLSQAEAGADWVMPGFTHLQTAQPVTWGHHMMAYVEMFGRDLSRV RDARKRMNESPLGAAALAGTSFPIDREMTASALGFDRPAANSLDAVSDRDFALEFLSVAS ISAVHLSRFAEELVIWSSAQFRFVTLSDRFSTGSSIMPQKKNPDAAELIRAKVGRIFGAN TALMMVMKGLPLAYSKDMQEDKEQVFDAADNWMLALAAMEGMVKDMTGNRAELAAAAGSG FSTATDLADWMVRVLKVPFRDAHHVTGTLVAMAESRGCDLPDLTLADMQGVHEGITEDIF SVLGVENSVNSRMSYGGTAPAQVRAQIARWQDVLSD