Protein Info for Psest_3401 in Pseudomonas stutzeri RCH2

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 TIGR00416: DNA repair protein RadA" amino acids 1 to 448 (448 residues), 659.3 bits, see alignment E=1.5e-202 PF18073: Zn_ribbon_LapB" amino acids 8 to 35 (28 residues), 39.8 bits, see alignment (E = 8.9e-14) PF06745: ATPase" amino acids 77 to 146 (70 residues), 39.3 bits, see alignment E=1.5e-13 PF13481: AAA_25" amino acids 79 to 220 (142 residues), 48.4 bits, see alignment E=2.8e-16 PF13541: ChlI" amino acids 351 to 427 (77 residues), 33.5 bits, see alignment E=1.1e-11 PF05362: Lon_C" amino acids 365 to 451 (87 residues), 30.8 bits, see alignment E=6.3e-11

Best Hits

Swiss-Prot: 91% identical to RADA_PSEAE: DNA repair protein RadA (radA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 97% identity to psa:PST_0943)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPG3 at UniProt or InterPro

Protein Sequence (453 amino acids)

>Psest_3401 DNA repair protein RadA (Pseudomonas stutzeri RCH2)
MAKAKRMYGCTECGSTFPKWAGQCGDCGAWNTLVETVIEGAAPPSGRAGWAGEQANIRTL
AEVSVEEVPRFSTASGELDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCAIAQHYPAL
YVTGEESQQQVAMRARRLDLPQDKLKVMTETCIESIIATARLEKPKVMVIDSIQTIFTEQ
LQSAPGGVAQVRESAALLVRFAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFEGE
SDGRLRLLRAVKNRFGAINELGVFGMTDKGLKEVTNPSAIFLTRAQEAVPGSVVMATWEG
TRPMLVEVQALVDTSHLANPRRVTLGLDQNRLAMLLAVLHRHGSIPTYDQDVFINVVGGV
KVLETAADLALMAAVISSLRNRPLDTDLLVFGEVGLSGEIRPVPSGQERLKEAAKHGFKR
AIVPKGNAPKEAPAGLQIIAVTRLEQALDALFE