Protein Info for Psest_3381 in Pseudomonas stutzeri RCH2

Annotation: integral membrane protein MviN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 transmembrane" amino acids 9 to 27 (19 residues), see Phobius details amino acids 34 to 52 (19 residues), see Phobius details amino acids 97 to 121 (25 residues), see Phobius details amino acids 140 to 160 (21 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 195 to 216 (22 residues), see Phobius details amino acids 236 to 258 (23 residues), see Phobius details amino acids 281 to 299 (19 residues), see Phobius details amino acids 320 to 346 (27 residues), see Phobius details amino acids 361 to 382 (22 residues), see Phobius details amino acids 391 to 410 (20 residues), see Phobius details amino acids 417 to 437 (21 residues), see Phobius details amino acids 448 to 469 (22 residues), see Phobius details amino acids 488 to 508 (21 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 10 to 504 (495 residues), 486.4 bits, see alignment E=5.1e-150 PF03023: MurJ" amino acids 35 to 477 (443 residues), 488 bits, see alignment E=2.4e-150 PF01554: MatE" amino acids 242 to 407 (166 residues), 27 bits, see alignment E=3.4e-10

Best Hits

Swiss-Prot: 48% identical to MURJ_ECOLI: Lipid II flippase MurJ (murJ) from Escherichia coli (strain K12)

KEGG orthology group: K03980, virulence factor (inferred from 98% identity to psa:PST_0962)

MetaCyc: 48% identical to lipid II flippase MurJ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-286

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPE1 at UniProt or InterPro

Protein Sequence (515 amino acids)

>Psest_3381 integral membrane protein MviN (Pseudomonas stutzeri RCH2)
MSEKTGKGGLLRSSAVVSVMTLLSRVLGMVRDMVVASYFGSGAAADAFFIAFKIPNFLRR
LFAEGAFAQAFVPVLSEYRTKRTLADVKQLVDRTAGMLGLILAGLTALGVLFAPYVVMVF
APGFHDDPAKMQLAGELLRITFPYLMLISLTAFTSGVLNSYGHFAVPGFTPVLLNVCMIA
SAVFLTPYFDQPIMALAWGVFIAGFAQLAFQLPYVAKLGLLPRPRVKRGDEGVRRIMLLM
VPALFGVSVSQINLLLDTVLASFLQTGSVSWLYYADRLSELPLGAFGIAIGTVILPSLSR
QHAGEDPKAFSATLDWALRMVLLVGVPAALALGILAEPMIASLFYYGAMSEEAVVQSARA
LQAYSLGVLAFMLIKVLAPGFFARQDLKTPVRVAVICMVANMVMNLILIWPLQHVGLALA
TSLSSMLNAVLLFWGLYKVGVYRPAPGWWSFGLRLAAACAAMVAVVWWLNVPAQEWFSWG
WQERALRLGILVCAGLAAFAGGLLLTGLRPRHLRH