Protein Info for Psest_3380 in Pseudomonas stutzeri RCH2

Annotation: riboflavin kinase/FMN adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 75% identical to RIBF_PSEFL: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Pseudomonas fluorescens

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 93% identity to psa:PST_0963)

MetaCyc: 55% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

flavin biosynthesis I (bacteria and plants) (8/9 steps found)
flavin salvage (2/2 steps found)
flavin biosynthesis III (fungi) (7/9 steps found)
5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
flavin biosynthesis II (archaea) (5/10 steps found)
adenosylcobalamin biosynthesis II (aerobic) (21/33 steps found)
roseoflavin biosynthesis (1/7 steps found)

KEGG Metabolic Maps

Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR56 at UniProt or InterPro

Protein Sequence (316 amino acids)

>Psest_3380 riboflavin kinase/FMN adenylyltransferase (Pseudomonas stutzeri RCH2)
MQLVRGLHNLRPRHRGCVATIGNFDGVHRGHQAILARLRERAAEMGLPSCVVIFEPQPRE
FFSPDKAPARLTRLREKLQLLSEQGVDLVLCLAFNRRLRELSAAEFVHATLVEGLGVQHL
EVGDDFRFGCDRAGDFDFLLKAGAAEGFSVEAATTIEVDGERVSSTRLRQVLAEGDLACA
EALLGRPFGITGRVMHGQKLGRQLGAPTANVQLKRRNTPLSGVFLVSFELDGKSLAGVAN
IGMRPTVESDGKPHLEVHLLDYQGDLYGRLVQVTFHRKLRDEQRFASLEALKTAIEADIA
AAREYWRASPLMTSQD