Protein Info for Psest_3355 in Pseudomonas stutzeri RCH2

Updated annotation (from data): fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components
Rationale: Specifically important for utilizing D-Fructose.
Original annotation: phosphoenolpyruvate-protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 960 PF00359: PTS_EIIA_2" amino acids 4 to 140 (137 residues), 89.1 bits, see alignment E=6.6e-29 PF00381: PTS-HPr" amino acids 285 to 366 (82 residues), 52.1 bits, see alignment E=1.4e-17 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 409 to 956 (548 residues), 497.8 bits, see alignment E=1.9e-153 PF05524: PEP-utilisers_N" amino acids 409 to 525 (117 residues), 89.1 bits, see alignment E=6.4e-29 PF00391: PEP-utilizers" amino acids 550 to 622 (73 residues), 73.4 bits, see alignment 2.5e-24 PF02896: PEP-utilizers_C" amino acids 653 to 932 (280 residues), 354.9 bits, see alignment E=7.9e-110

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 91% identity to psa:PST_0988)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) / Fructose-specific phosphocarrier protein HPr (EC 2.7.1.69) / PTS system, fructose-specific IIA component (EC 2.7.1.69)" in subsystem Fructose utilization or Fructose and Mannose Inducible PTS or Mannitol Utilization (EC 2.7.1.69, EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR32 at UniProt or InterPro

Protein Sequence (960 amino acids)

>Psest_3355 fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (Pseudomonas stutzeri RCH2)
MLELNAQHIHMHQAAADKPAALALLGEVLVADGLVAAGYLEGLRAREAQGSTFLGQGIAI
PHGTPETRDQVFTTGVRLLHFPAGVDWGNGQLVYLAIGIAARSDEHLRLLQLLTRALGEG
DLSEGLQQAESPEAIIGLLQGAPQALALDGELVSLGVAADDFDELAWQGVKLLKRAQCVE
PGFNASLPLGQSLPLGDGLWWLSSEQSVQRPGLAFVTPVSNLEHQGQPLNGLFVLASMGE
AHQAMLERLCNLLIEGRGQELSQATSSRTVLEALGGDVPADWPSAQVPLANAHGLHARPA
KVLTEIAQAFEGEIRVRLAGTESAGVSVKSLSKLLAMGAHRGQLLEFMAEPAIASDALPA
LVRAVEEGLGEEVEPLPAPGEASEPPVTAQGPAESAIDQAALRAGDQVNGIAASPGIAIG
PVLVRKPQVIDYPKRGESPVIELQRLDAALDKVHADIGTLIDESQVASIRDIFTTHQAML
KDPALREEVQVRLQKGLSAEAAWMEEIESAAQQQEALHDKLLAERAADLRDVGRRVLACL
TGVEAEQAPDEPYILVMDEVAPSDVATLNAQRVAGILTAGGGATSHSAIIARALGIPAIV
GAGPGVLGLARNTLLLLDGERGELLVAPSGAQLEQARSERAAREERKHLANERRMDAAVT
RDGHPVEIAANIGAAGETPEAVAMGAEGIGLLRTELVFMNHSQAPNQATQEAEYRRVLEA
LEGRPLVVRTLDVGGDKPLPYWPMPAEENPFLGVRGIRLSLQRPDILETQLRALLASADG
RPLRIMFPMVGNIDEWRTAKAMVDRLRVELPVADLQVGIMIEIPSAALIAPVLAQEVDFF
SIGTNDLTQYTLAIDRGHPTLSGQADGLHPAVLRLIGMTVEAAHAHGKWVGVCGELAADA
LAVPLLVGLGVDELSVSARSIALVKARVRELDFAACQRLAQQALMLPGAHEVRAFVGEHC