Protein Info for Psest_3353 in Pseudomonas stutzeri RCH2

Annotation: PTS system, fructose-specific, IIB component/PTS system, fructose subfamily, IIC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 transmembrane" amino acids 248 to 270 (23 residues), see Phobius details amino acids 290 to 308 (19 residues), see Phobius details amino acids 320 to 343 (24 residues), see Phobius details amino acids 363 to 383 (21 residues), see Phobius details amino acids 403 to 422 (20 residues), see Phobius details amino acids 442 to 465 (24 residues), see Phobius details amino acids 477 to 497 (21 residues), see Phobius details amino acids 503 to 525 (23 residues), see Phobius details amino acids 544 to 565 (22 residues), see Phobius details PF02302: PTS_IIB" amino acids 5 to 93 (89 residues), 48.7 bits, see alignment E=9.6e-17 amino acids 121 to 211 (91 residues), 72.8 bits, see alignment E=3e-24 TIGR00829: PTS system, Fru family, IIB component" amino acids 121 to 204 (84 residues), 110.5 bits, see alignment E=3.7e-36 TIGR01427: PTS system, Fru family, IIC component" amino acids 229 to 566 (338 residues), 472.3 bits, see alignment E=1.1e-145 PF02378: PTS_EIIC" amino acids 247 to 510 (264 residues), 71 bits, see alignment E=9.9e-24

Best Hits

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 92% identity to psa:PST_0990)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GM60 at UniProt or InterPro

Protein Sequence (579 amino acids)

>Psest_3353 PTS system, fructose-specific, IIB component/PTS system, fructose subfamily, IIC component (Pseudomonas stutzeri RCH2)
MNLLIVTACPNGMVTSVLTSRLLEAAAHRLGWSTAVEVHDPKAIGSPLTPAQIANADLVV
VVKTGPLSLQRFVGKRVAQSTPSEALLDPEAFLRSAADTASELQQADEAEAAHTSGKPKL
VAVTACPTGVAHTFMAAEALQQAAIRKGYDLQVETRGSVGARNVLEADVIAAADVVLLAA
DIEVDVARFAGKRVFRCGTGVALKQPEATLDRALEEGAVLSGNAVATSASGEKKGEKTGV
YKHMLTGVSYMLPMVVAGGLLIALSFVFGIEAFKEEGTLAAALMKIGGETAFQLMVPLLA
GYIAYSIADRPGLAPGMIGGLLAGTLGAGFIGGIIAGFVAGYAAKAVSRWIPLPASIESL
KPILIIPLLASLVTGLVMIYIVGTPVAKLLAGLTEFLDTMGTSNAILLGLLLGTMMCVDL
GGPVNKAAYAFSVGLLASQSYAPMAATMAAGMVPPIGMGIATLIARRKFAQTEREAGKAA
LVLGCCFISEGAIPFAAKDPLRVIPASIAGGALTGALSMAFGAKLLAPHGGLFVLLIPNA
INHALLYLVAILAGSLVTGVIYAVIKQSEPQGLMVQAQT