Protein Info for Psest_0328 in Pseudomonas stutzeri RCH2

Annotation: transketolase, bacterial and yeast

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00456: Transketolase_N
  • amino acids 5 to 333 (329 residues), 551.1 bits, E=2.1e-169
• TIGR00232: transketolase
  • amino acids 6 to 663 (658 residues), 1024 bits, E=0
• PF02779: Transket_pyr
  • amino acids 353 to 524 (172 residues), 181.3 bits, E=3.3e-57
• PF22613: Transketolase_C_1
  • amino acids 539 to 651 (113 residues), 142 bits, E=2.3e-45
• PF02780: Transketolase_C
  • amino acids 549 to 655 (107 residues), 64.1 bits, E=2.9e-21

Best Hits

Swiss-Prot: 74% identical to TKT1_ECOLI: Transketolase 1 (tktA) from Escherichia coli (strain K12)

KEGG orthology group: K00615, transketolase [EC: 2.2.1.1] (inferred from 97% identity to psa:PST_3920)

MetaCyc: 74% identical to transketolase 1 (Escherichia coli K-12 substr. MG1655)
Transketolase. [EC: 2.2.1.1]; 2.2.1.1 [EC: 2.2.1.1]

Predicted SEED Role

"Transketolase (EC 2.2.1.1)" in subsystem Calvin-Benson cycle or Pentose phosphate pathway (EC 2.2.1.1)

MetaCyc Pathways

• Bifidobacterium shunt (15/15 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• pentose phosphate pathway (7/8 steps found)
• pentose phosphate pathway (partial) (3/3 steps found)
• Rubisco shunt (8/10 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• oxygenic photosynthesis (11/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Carbon fixation in photosynthetic organisms
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.1

Sequence Analysis Tools

See L0GI01 at UniProt or InterPro

Protein Sequence (665 amino acids)

>Psest_0328 transketolase, bacterial and yeast (Pseudomonas stutzeri RCH2)
MPSRRERANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWRDHLKHSPTNPQWADRDRF
VLSNGHGSMLIYSLLHLTGYDLSIDDLKNFRQLHSKTPGHPEFGYTAGVETTTGPLGQGL
ANAVGFAVAEKVMAAQFNRPGHNIVDHNTYVFLGDGCMMEGISHEVCSLAGTLGLNKLIA
FYDDNGISIDGEVHGWFTDDTPRRFEAYGWQVIRNVDGHDADEIQMAIETARKSDRPTLI
CCKTIIGFGSPNKQGKEESHGAALGDAEIALTREALGWKYGPFEIPAEIYAEWDAKQKGA
EAENEWNKRFAAYEAEFPALASEFKRRMAGELPADFAEKASEFIREVANKGETIASRKAS
QNCLNAFGPLLPELLGGSADLAGSNLTLWKGCKPVVAEDASGNYMYYGVREFGMAAIMNG
VALHGGLIPYGATFLMFMEYARNAVRMSALMKQRVLYVFTHDSIGLGEDGPTHQPIEQLT
SLRTTPNLDTWRPADTVESAVAWKHALERKDGPSALIFSRQNLPFHVRDNETEAAIARGG
YILKNCAGEPELILIATGSEVSLAVQAADKLTEQGRKVRVVSMPCTSVFDSQDAAWKQHV
LPVEVGARIAIEAAHADYWYKYVGLDGRIIGMTTYGESAPANQLFEEFGFTVDNILGIAE
ELLED