Protein Info for Psest_3330 in Pseudomonas stutzeri RCH2
Annotation: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to GATB_PSEU5: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (gatB) from Pseudomonas stutzeri (strain A1501)
KEGG orthology group: K02434, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit B [EC: 6.3.5.6 6.3.5.7] (inferred from 97% identity to psa:PST_1012)MetaCyc: 49% identical to glutamyl-tRNA(Gln) amidotransferase, B subunit (Synechococcus elongatus PCC 7942 = FACHB-805)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]; Asparaginyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7, 6.3.5.6]
Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit B (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit B (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GP81 at UniProt or InterPro
Protein Sequence (481 amino acids)
>Psest_3330 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (Pseudomonas stutzeri RCH2) MQWETVIGLEIHAQLATQSKIFSGSATTFGAEPNTQASLVDLGMPGTLPVLNAEAVRMAC KFGLAIEAEIAPKNVFARKNYFYPDLPKGYQTSQMDHPIVGKGYLDITLEDGSSRRIGIT RAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDIRSAKEAVAYVKAIHALVRYL GICDGNMAEGSLRCDCNVSVRPKGQAEFGTRAEIKNVNSFRFIEKAINHEVQRQIELIED GGKVVQETRLYDPNKDETRSMRSKEEANDYRYFPCPDLLPVVIEQSFLDEVRASLPELPV QKRERFESEFGLSAYDATVLAASRELADYFEQVNATCGDAKLAANWVMGELSSLLNKDGL DIEQSPVTAEQLGGMILRIKDDTISGKIAKMVFEAMAAGEGSADEIIEKKGLKQVTDSGA IEKMLDEVLAANAEQVEQYRASDETKRGKMFGFFVGQAMKASKGKANPGQVNQLLKKKLE G