Protein Info for Psest_3329 in Pseudomonas stutzeri RCH2

Annotation: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 86% identical to GATA_PSEA7: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 98% identity to psa:PST_1013)

MetaCyc: 52% identical to glutamyl-tRNAGln amidotransferase subunit A (Bacillus subtilis)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

L-citrulline biosynthesis (7/8 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
superpathway of L-citrulline metabolism (9/12 steps found)
L-glutamine degradation I (1/1 steps found)
L-glutamate and L-glutamine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR03 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Psest_3329 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (Pseudomonas stutzeri RCH2)
MHNLTLAEIARNLAEKRFSAEELTRTLLARIEQLDPQLNSFISVTDELAIAQAKAADARR
AAGESGALLGAPIGHKDLFCTQGIRTSCGSKILDNFKAPYNATVVERLAAAGTVTLGKLN
MDEFAMGSANESSYYGPVKNPWDPSRVPGGSSGGSAAAIAARLLPAATGTDTGGSIRQPA
ALTNLTGLKPTYGRVSRWGMVAYASSLDQGGPMARTAEDCALLLSAMAGFDAKDSTSVDQ
PLDDYLAALSQPLAGLRIGLPKEYFGAGLDPKIADAVMASVEELKKLGATVKDISLPNMQ
HAIPSYYVIAPAEASSNLSRFDGVRFGYRCENPVDLTDLYKRSRAEGFGDEVKRRIMVGT
YALSAGYYDAYYLKAQKIRRLIKQDFVTAFEQVDLILGPTTPNLAWKLGEKNADPVSAYL
EDIYTITANLAGIPGLSMPAGFIDGLPVGVQLLAPYFQEARLLNVAHQYQQVTDWHARAP
AGF