Protein Info for Psest_3317 in Pseudomonas stutzeri RCH2

Annotation: Phosphotransferase System HPr (HPr) Family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 50 75 90 TIGR01003: phosphocarrier, HPr family" amino acids 1 to 82 (82 residues), 89.9 bits, see alignment E=3.9e-30 PF00381: PTS-HPr" amino acids 4 to 83 (80 residues), 100.5 bits, see alignment E=2.2e-33

Best Hits

Swiss-Prot: 77% identical to PTHP_PSEAE: Phosphocarrier protein HPr (ptsH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K11189, phosphocarrier protein (inferred from 91% identity to psa:PST_1027)

Predicted SEED Role

"Phosphocarrier protein, nitrogen regulation associated"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GM25 at UniProt or InterPro

Protein Sequence (90 amino acids)

>Psest_3317 Phosphotransferase System HPr (HPr) Family (Pseudomonas stutzeri RCH2)
MPAREVTIINKLGLHARAAAKFVAVANRFPCKIRVGRCPDTLVDGKSIMAVMMLAASKGT
SVHLHTEGEQEDDALNALIALIDDYFEEGE