Protein Info for PGA1_c33000 in Phaeobacter inhibens DSM 17395

Annotation: polyribonucleotide nucleotidyltransferase Pnp

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 712 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 10 to 693 (684 residues), 1024.5 bits, see alignment E=0 PF01138: RNase_PH" amino acids 16 to 144 (129 residues), 104 bits, see alignment E=2e-33 amino acids 327 to 459 (133 residues), 88.6 bits, see alignment E=1.2e-28 PF03725: RNase_PH_C" amino acids 147 to 216 (70 residues), 40 bits, see alignment E=7.5e-14 amino acids 463 to 532 (70 residues), 32.2 bits, see alignment E=2e-11 PF03726: PNPase" amino acids 246 to 323 (78 residues), 51.2 bits, see alignment E=3.6e-17 PF00013: KH_1" amino acids 559 to 616 (58 residues), 43.7 bits, see alignment 4.7e-15 PF00575: S1" amino acids 621 to 693 (73 residues), 72 bits, see alignment E=1.1e-23

Best Hits

Swiss-Prot: 90% identical to PNP_RUEST: Polyribonucleotide nucleotidyltransferase (pnp) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 90% identity to sil:SPO3389)

MetaCyc: 57% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7ERH4 at UniProt or InterPro

Protein Sequence (712 amino acids)

>PGA1_c33000 polyribonucleotide nucleotidyltransferase Pnp (Phaeobacter inhibens DSM 17395)
MFNVTKKSMQWGEETLTLETGKVARQADGTVIATLGETSVMANVTFARQQKPGQDFFPLT
VHYQEKYYAAGKVPGGFFKREARPTEKETLTARLIDRPIRPLFVPGFKNEVLVMCTVLSH
DLVNDPDMVAMIAASAALTLSGAPFMGPIAAARVGFEGGEYVLNPTVDDMQDLRLNPDQR
LDLVVAGTKDAVMMVESEAYELTEAEMLGAVKFAHEQIQPVIDLIISLAEEAAKEPFDFQ
PADYSELFAAVKAAGEEQMRAAFAITDKQERTAAVAAAREAIKTSLSEEQLEDANLGSAL
KKLEAGILRGDVVKTGKRIDGRSTTDVRAIESETGMLPRTHGSALFTRGETQALAVTTLG
TGDDEQFIDALHGNFKSNFLLHYNFPPYSVGEVGRVGSPGRREIGHGKLAWRALQAVLPA
PTDFPYTIRVVSEITESNGSSSMASVCGGSLSMMDAGVPLKAPVAGVAMGLILEDDGSYA
ILSDILGDEDHLGDMDFKVAGTDKGITSLQMDIKVAGITPEIMEKALDQAKDGRLHILGE
MAKALSETNAFSVHAPRIETMQIPTDKIREVIGSGGKVIREIVETSGAKVDINDDGVIKI
ASADGTAIQKAYDMIHSIVAEPEEGAVYTGKVVKIVDFGAFVNFFGKRDGLVHVSQIENR
RLNHPSDVLKEGQEVKVKLLGFDDRGKVRLSMKIVDQETGEEIKAEKKEDAE