Protein Info for PGA1_c32860 in Phaeobacter inhibens DSM 17395

Annotation: bifunctional purine biosynthesis protein PurH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 8 to 529 (522 residues), 692.3 bits, E=2.3e-212
• PF02142: MGS
  • amino acids 19 to 132 (114 residues), 84.5 bits, E=4.6e-28
• PF01808: AICARFT_IMPCHas
  • amino acids 138 to 452 (315 residues), 409.5 bits, E=1.1e-126

Best Hits

Swiss-Prot: 88% identical to PUR9_RUEST: Bifunctional purine biosynthesis protein PurH (purH) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 88% identity to sit:TM1040_0091)

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Sequence Analysis Tools

See I7E525 at UniProt or InterPro

Protein Sequence (529 amino acids)

>PGA1_c32860 bifunctional purine biosynthesis protein PurH (Phaeobacter inhibens DSM 17395)
MTDLHPVRRALLSVSDKTGLIDLGKALAARGIELLSTGGTAKALREAGLTVKDVSEITGF
PEMMDGRVKTLHPMVHGGLLALRDNDTHVAEMDEHGIGAIDLLVVNLYPFEETVAKGADY
DTCIENIDIGGPAMIRAASKNHLFVNVVVDVEDYEPLLAELDKNDGQTSYGFRQWLSQNA
YARTAAYDAAVSNWMAGALNLEAPRRRAVAGQLAQTLRYGENPHQQAAFYLDGSARAGVA
TAQQVQGKELSYNNINDTDAAFELVSEFALEDGPAVAIIKHANPCGVARGETLKDAYQKA
FDCDRTSAFGGIVALNQPLDAETAAEIVLIFTEVVIAPGASDEAKAIFASKKNLRLLLTD
GLPNPQDTGLTTRQVSGGMLVQDKDVGHRAMDDLKVVTEKAPTEEQMQDLLFAWKVAKHV
KSNAIVYVKSGQTVGVGAGQMSRLDSATIAGIKAQRMADALELPESLAKGSAVASDAFFP
FADGLLEAAANGATCVIQPGGSMRDDEVIKAANDAGLAMVFTGMRHFRH