Protein Info for Psest_0324 in Pseudomonas stutzeri RCH2

Annotation: Methylase involved in ubiquinone/menaquinone biosynthesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 PF12840: HTH_20" amino acids 21 to 67 (47 residues), 33.5 bits, see alignment 1.5e-11 PF01022: HTH_5" amino acids 23 to 65 (43 residues), 28.9 bits, see alignment 3.9e-10 PF13489: Methyltransf_23" amino acids 146 to 297 (152 residues), 60.9 bits, see alignment E=5.9e-20 PF00891: Methyltransf_2" amino acids 152 to 265 (114 residues), 25 bits, see alignment E=5e-09 PF01209: Ubie_methyltran" amino acids 160 to 266 (107 residues), 27.1 bits, see alignment E=1.2e-09 PF13847: Methyltransf_31" amino acids 162 to 273 (112 residues), 57.6 bits, see alignment E=5.3e-19 PF13649: Methyltransf_25" amino acids 166 to 256 (91 residues), 59.9 bits, see alignment E=1.5e-19 PF08242: Methyltransf_12" amino acids 166 to 258 (93 residues), 53.8 bits, see alignment E=1.2e-17 PF08241: Methyltransf_11" amino acids 166 to 259 (94 residues), 62.4 bits, see alignment E=2.4e-20

Best Hits

KEGG orthology group: K03892, ArsR family transcriptional regulator (inferred from 93% identity to psa:PST_3925)

Predicted SEED Role

"Transcriptional regulator, ArsR family / Methyltransferase fusion"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHP5 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Psest_0324 Methylase involved in ubiquinone/menaquinone biosynthesis (Pseudomonas stutzeri RCH2)
MPQISHDTSDQLASLCKAGGDALRLKVLRALSSDSFGVLELAQIFAIGQSGMSHHLKVLT
QAGLLATRREGNAVFYRRSLPQADSLGGRLHAALLEEADRLELPREIEARIAAVHTQRSA
ASEDFFARMADSFQARQDLIAGLPQYRDSVLALLDALNFDNSATALEVGPGDGSFLPELA
RRFARVVALDNSPAMLDLARARCEEAGLENVELKLADALHDDCPPADCVILNMVLHHLAA
PGEAMKQLARLVNAGGSLLVTELCSHNQSWAREACGDLWLGFEQDDLARWADAAGLTPGE
SLYIGLKNGFQIQARYFSRLAPDSRLTHR