Protein Info for HP15_316 in Marinobacter adhaerens HP15

Annotation: protein containing DNA primase, DnaG/TOPRIM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 TIGR01391: DNA primase" amino acids 5 to 409 (405 residues), 451 bits, see alignment E=2e-139 PF01807: zf-CHC2" amino acids 6 to 100 (95 residues), 133.1 bits, see alignment E=9e-43 PF08275: DNAG_N" amino acids 123 to 246 (124 residues), 125.7 bits, see alignment E=4.4e-40 PF13662: Toprim_4" amino acids 254 to 321 (68 residues), 60.5 bits, see alignment E=5.2e-20 PF13362: Toprim_3" amino acids 255 to 350 (96 residues), 34.1 bits, see alignment E=1.1e-11 PF01751: Toprim" amino acids 255 to 328 (74 residues), 48.9 bits, see alignment E=2.1e-16 PF13155: Toprim_2" amino acids 257 to 343 (87 residues), 66.3 bits, see alignment E=9.3e-22

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 85% identity to maq:Maqu_0659)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PL38 at UniProt or InterPro

Protein Sequence (575 amino acids)

>HP15_316 protein containing DNA primase, DnaG/TOPRIM (Marinobacter adhaerens HP15)
MSGLIPQQFVENLLDRIDLAELIGSRITLKKAGANYKACCPFHDEKTPSFNVRPDKGFYH
CFGCGAHGDAISFVREFEGLGFTEAVEELAKRVGLEVPYDRAAKQEIQQARTLTDALDFA
SRFYQSALKGQQGAYARDYLQQRGLDNAIIEQYQVGYAPSTGTALFDVASKDLQGPLIET
GTVSDKYGKPRDLFRNRVMFPIRNSRGRTVAFGGRTLGDDKAKYINSPESDVFHKSREIY
GLFEAKQAIRQLDKLLVVEGYMDVIALAQHGIHYAVATLGTATNQDSLTALLKQVRHIVF
CFDGDQAGFRAADRAMENALELMADGLHLQFLMLPQGEDPDTLVRKEGPEAFQKRIEGAT
PLSRYLFDRQSEGLDLQLPEHRGELRARVEPLLNKMPRSTLRDAMWHEMLRLCGGRNQWQ
SRQQGQKGRWNGERRDRVSEDRIDVKLSKDSILCLALLEAPDLATEVTELARSSRQFNQA
RNFAEWILANDIRDRKTLVRTLALDTGARERFYHLFDGIEHIPARESTLAAARELLSPNE
EASRQQRLATLLRNLSNLTSEERQELRALSSGTGD