Protein Info for HP15_3081 in Marinobacter adhaerens HP15

Annotation: chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 TIGR02350: chaperone protein DnaK" amino acids 3 to 603 (601 residues), 961 bits, see alignment E=1.1e-293 PF00012: HSP70" amino acids 4 to 603 (600 residues), 897.9 bits, see alignment E=2.7e-274 PF06723: MreB_Mbl" amino acids 115 to 378 (264 residues), 43.8 bits, see alignment E=1.6e-15

Best Hits

Swiss-Prot: 96% identical to DNAK_MARHV: Chaperone protein DnaK (dnaK) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 96% identity to maq:Maqu_3362)

MetaCyc: 77% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PPH4 at UniProt or InterPro

Protein Sequence (641 amino acids)

>HP15_3081 chaperone protein DnaK (Marinobacter adhaerens HP15)
MSKIIGIDLGTTNSCVAIMDGDKVKVIENAEGDRTTPSIIAYTDDGETLVGQSAKRQAVT
NPNNTLYAIKRLIGRRFEDDVVQKDIKMVPYKIAKADNGDAWVEVKGEKMAPPQVSAEVL
KKMKKTAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGKIAGLEVKRIINEPTAAALAYG
LDKKSGDRTVAVYDLGGGTFDLSIIEIADVDGEHQFEVLATNGDTFLGGEDFDLKIIEYL
ADQFKKDSGIDLRGDSLAMQRLKEAGEKAKIELSSSQQTDVNLPYITADASGPKHMNVKL
TRAKLESLVEDLVQRSLEPCKVALQDAGMKAGEVDEVILVGGQTRMPLVQEKVKEFFGKE
ARKDVNPDEAVAMGAAIQAAVLSGDVKDVLLLDVTPLTLGIETMGGVATPLIDKNTTIPT
KKSQTFSTADDNQTAVTIHVVQGERKQAAQNKSLGRFDLADIPPAARGVPQIEVTFDIDA
NGILNVSAKDKATGKEQSIVIKASSGLNDDEIEKMVQDAEANAEEDRKFEELVQVRNQGD
AMVHAVRKTLNEAGDKVSDSEKESIEASIKELETALEGSDKEDIEAKTQKLTEVSSELAQ
KMYADQADQTQQAGGQEEAQGQKSDDAVDAEFEEVKDDDKR