Protein Info for Psest_3174 in Pseudomonas stutzeri RCH2
Annotation: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to ALGA_PSEAE: Alginate biosynthesis protein AlgA (algA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 96% identity to psa:PST_1169)MetaCyc: 80% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]
Predicted SEED Role
"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) / Mannose-6-phosphate isomerase (EC 5.3.1.8)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22, EC 5.3.1.8)
MetaCyc Pathways
- colanic acid building blocks biosynthesis (10/11 steps found)
- GDP-mannose biosynthesis (4/4 steps found)
- β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
- D-mannose degradation I (1/2 steps found)
- D-mannose degradation II (1/2 steps found)
- mannitol degradation II (2/4 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
- mannitol biosynthesis (1/3 steps found)
- 1,5-anhydrofructose degradation (2/5 steps found)
- β-(1,4)-mannan degradation (2/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.13, 2.7.7.22
Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GQT1 at UniProt or InterPro
Protein Sequence (481 amino acids)
>Psest_3174 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (Pseudomonas stutzeri RCH2) MIPVILSGGSGSRLWPLSRKSFPKQFLALTGEQTLFQQTVERLAFDGLQQPLLVCNQEHR FIVKEQLAARKLGVQGLLLEPFGRNTAPAIGIAAMKLIEEGRDELLLVLPADHVIEDQKA FQRSLALATNAAENGEMVLFGVPPTRPETGFGYIKASQDANAGLPDGIKRVAQFVEKPDE ARAQSYVESGDYFWNSGMFLFRASVFLDELKKHDPDIYDTCWVALERSIKNGDEVLIDPA TFACCPDNSIDYAVMEKTQLACVVPMSAGWNDVGSWSSIWDVHQKDENGNVLKGDVIAED SRNCLVHGNGKLVTVLGLDDIVVVETKDAMMVAHKDKVQDVKKLVSKLDALERSETKNHC AVYRPWGWYDSVDMGGRFQVKRICVNPGASLSLQMHHHRAEHWIVVSGTAQVTCNEKTFL LTENQSTYIPITSVHRLANPGKIPLEIIEVQSGSYLGEDDIERFDDVYGRAEQSNEAKVA R