Protein Info for Psest_3165 in Pseudomonas stutzeri RCH2

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 PF00027: cNMP_binding" amino acids 14 to 89 (76 residues), 30.8 bits, see alignment E=2.3e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 122 to 284 (163 residues), 153.2 bits, see alignment E=2.6e-49 PF00990: GGDEF" amino acids 125 to 283 (159 residues), 141 bits, see alignment E=3e-45

Best Hits

KEGG orthology group: None (inferred from 61% identity to pmk:MDS_3829)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQI8 at UniProt or InterPro

Protein Sequence (286 amino acids)

>Psest_3165 diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MLTALAEKLETCEVDAEELLLSPHTLNRHLYMLVRGQLSVQLASRDNPPVRYVEVNDCVG
EVSFCDHRPPSAYVIASEPSRVVRLHSRELPLLTQSPQLMLNLIELLCDRVRLTDQLIIN
SEHNANIDMLTGVFNRRWLEHIFERESARCAFSGQPLCMLMIDVDEFKPYNDRHGHIAGD
HALCQVARLLGTQLRPKDSLVRFGGEEFVTLLPELDLPAARLIAERLRQRVAQNDTIATP
IGSLPGVTISVGLARMRPSDTLEDLIGRADKALYLAKAQGRNRVCD