Protein Info for Psest_3118 in Pseudomonas stutzeri RCH2

Annotation: endonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 212 TIGR01083: endonuclease III" amino acids 4 to 194 (191 residues), 283.5 bits, see alignment E=3.7e-89 PF00730: HhH-GPD" amino acids 34 to 168 (135 residues), 76.8 bits, see alignment E=2.4e-25 PF00633: HHH" amino acids 100 to 127 (28 residues), 38.2 bits, see alignment (E = 1.2e-13) PF10576: EndIII_4Fe-2S" amino acids 187 to 203 (17 residues), 22 bits, see alignment (E = 2.5e-08)

Best Hits

Swiss-Prot: 73% identical to END3_HAEIN: Endonuclease III (nth) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K10773, endonuclease III [EC: 4.2.99.18] (inferred from 96% identity to psa:PST_1206)

MetaCyc: 71% identical to endonuclease III (Escherichia coli K-12 substr. MG1655)
DNA-(apurinic or apyrimidinic site) lyase. [EC: 4.2.99.18]; 4.2.99.18 [EC: 4.2.99.18]; 4.2.99.18 [EC: 4.2.99.18]

Predicted SEED Role

"Endonuclease III (EC 4.2.99.18)" in subsystem Control of cell elongation - division cycle in Bacilli or DNA Repair Base Excision (EC 4.2.99.18)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQN0 at UniProt or InterPro

Protein Sequence (212 amino acids)

>Psest_3118 endonuclease III (Pseudomonas stutzeri RCH2)
MNSEKRREIFRRLHEDNPEPKTELAYNTPFELLIAVILSAQATDVGVNKATAKLYPVANT
PEAIYALGYDGLCEYIRTIGLYPSKAKNVIETCRILIEKHGGQVPDNREDLEALPGVGRK
TANVVLNTAFRQFTMAVDTHIFRVSNRTGIAPGKNVLEVERKLIRFVPKEYLLDAHHWLI
LHGRYVCKARKPQCGSCRIEDLCEYKHKTSDD