Protein Info for Psest_3101 in Pseudomonas stutzeri RCH2

Annotation: L-aspartate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR00551: L-aspartate oxidase" amino acids 7 to 520 (514 residues), 665.3 bits, see alignment E=2.8e-204 PF00890: FAD_binding_2" amino acids 8 to 392 (385 residues), 350.8 bits, see alignment E=2.6e-108 PF02910: Succ_DH_flav_C" amino acids 441 to 521 (81 residues), 49.6 bits, see alignment E=8e-17

Best Hits

Swiss-Prot: 85% identical to NADB_PSEAE: L-aspartate oxidase (nadB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00278, L-aspartate oxidase [EC: 1.4.3.16] (inferred from 96% identity to psa:PST_1222)

MetaCyc: 65% identical to L-aspartate oxidase (Escherichia coli K-12 substr. MG1655)
1.5.98.-; L-aspartate oxidase. [EC: 1.4.3.16]

Predicted SEED Role

"L-aspartate oxidase (EC 1.4.3.16)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 1.4.3.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GP85 at UniProt or InterPro

Protein Sequence (538 amino acids)

>Psest_3101 L-aspartate oxidase (Pseudomonas stutzeri RCH2)
MNQVLRYDVLVIGSGAAGLTLALNLPADLRIAVLSKGDLSNGSTYWAQGGVAAVLDTTDT
VESHVADTLIAGGGLCHEDAVRFTVEHSNEAIQWLIEQGVPFTRDDAPDREDGSFEFHLT
REGGHSHRRIIHAADATGAAIFNTLLGQARQKDNIELLQQRVAVDLITEHKLGLPGRRCL
GAYVLNRNTGEVETFQSRFVVLATGGAAKVYLYTSNPDSACGDGIAMAWRAGCRVGNLEF
NQFHPTCLYHPQAKSFLVTEALRGEGALLKLPNGERFMPRFDAREELAPRDIVARAIDHE
MKRLGIDCVYLDISHKPADFIKSHFPTVYERCLEYGIDITSQPIPVVPAAHYTCGGVVVD
QAGRTDIPGLYAIGETSFTGLHGANRMASNSLLECFVYGRAAAQDILRELPDITDSTTLP
AWDASQVTDSDEDVIIAHNWDELRRFMWDYVGIVRTNKRLMRAQHRVRLLLSEIDEFYSN
YKVSRDLLELRNLALVADLMIRSAMERKESRGLHYTLDYPQLLPQALDTILAPPTFAD