Protein Info for Psest_3095 in Pseudomonas stutzeri RCH2

Annotation: GTP-binding protein LepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 6 to 185 (180 residues), 184.3 bits, E=4.7e-58
• TIGR01393: elongation factor 4
  • amino acids 6 to 598 (593 residues), 968.4 bits, E=1.2e-295
• TIGR00231: small GTP-binding protein domain
  • amino acids 9 to 176 (168 residues), 68.1 bits, E=8.1e-23
• PF00071: Ras
  • amino acids 68 to 182 (115 residues), 21.8 bits, E=3.1e-08
• PF03144: GTP_EFTU_D2
  • amino acids 208 to 278 (71 residues), 36.8 bits, E=1.1e-12
• PF00679: EFG_C
  • amino acids 403 to 487 (85 residues), 66 bits, E=6.2e-22
• PF06421: LepA_C
  • amino acids 490 to 596 (107 residues), 167.2 bits, E=2.7e-53

Best Hits

Swiss-Prot: 94% identical to LEPA_PSEU5: Elongation factor 4 (lepA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 94% identity to psa:PST_1228)

MetaCyc: 74% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See L0GNM4 at UniProt or InterPro

Protein Sequence (599 amino acids)

>Psest_3095 GTP-binding protein LepA (Pseudomonas stutzeri RCH2)
MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLTEREMEAQVLDSMDLERERGITIKAH
SVTLYYPARDGKTYQLNFIDTPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCY
TAIEQGLEVMPVLNKMDLPQADPEKVKDEIEHIIGIDATDAVACSAKSGMGVDEVLERLV
AVIPPPTGDIEAPLQALIIDSWFDNYLGVVSLVRVRHGRIKKGDKVLVKSTGKVHQVDSV
GVFNPKHTATADLKAGEVGFIIAGIKDIHGAPVGDTLTLSSTPDVEMLPGFKRVKPQVYA
GLFPVSSDDFEDFREALQKLTLNDAALQYEPESSDALGFGFRIGFLGMLHMEIIQERLER
EYDLDLITTAPTVVYEILLKNGDTIYVDSPSKLPDMSSIEDMREPIVRANILVPQEHLGN
VITLCIEKRGVQRDLQFLGTQVQVRYDLPMSEVVLDFFDRLKSCSRGYASLDYSFECFQS
ANLTRLDILINGDKVDALALIVHRDNAHYKGRILVEKMKELIPRQMFDVAIQAALGGQIV
ARSTVKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLKVDS