Protein Info for HP15_300 in Marinobacter adhaerens HP15

Annotation: ribonuclease PH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 238 TIGR01966: ribonuclease PH" amino acids 2 to 236 (235 residues), 377.8 bits, see alignment E=8e-118 PF01138: RNase_PH" amino acids 11 to 140 (130 residues), 106.1 bits, see alignment E=1.9e-34 PF03725: RNase_PH_C" amino acids 158 to 224 (67 residues), 45.5 bits, see alignment E=5.9e-16

Best Hits

Swiss-Prot: 92% identical to RNPH_MARHV: Ribonuclease PH (rph) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K00989, ribonuclease PH [EC: 2.7.7.56] (inferred from 92% identity to maq:Maqu_0548)

Predicted SEED Role

"Ribonuclease PH (EC 2.7.7.56)" in subsystem Heat shock dnaK gene cluster extended or tRNA processing (EC 2.7.7.56)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.56

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PL22 at UniProt or InterPro

Protein Sequence (238 amino acids)

>HP15_300 ribonuclease PH (Marinobacter adhaerens HP15)
MRPSGRTPEQPRDVRITRNYTRHAEGSVLVEFGDTKVICTASVENKVPPFLRGEGKGWIT
AEYGMLPRSTGSRMGREAARGKQGGRTVEIQRLIGRSLRAAVDLTALGEHSITIDCDVIQ
ADGGTRTAAITGGCVALVDALNHLVADKRLKKSPLKQMVAALSVGVYKGTPVVDLDYPED
SEAETDMNVIMTDQGGFIEIQGTAEGAPFDQSELDSMLTLAKQGIEQLFEIQKAALEA