Protein Info for PGA1_c30470 in Phaeobacter inhibens DSM 17395

Annotation: mandelate racemase / muconate lactonizing enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF02746: MR_MLE_N" amino acids 4 to 107 (104 residues), 53.3 bits, see alignment E=3.1e-18 PF13378: MR_MLE_C" amino acids 130 to 283 (154 residues), 96.9 bits, see alignment E=1.4e-31

Best Hits

Swiss-Prot: 47% identical to AEEP_ECOLI: L-Ala-D/L-Glu epimerase (ycjG) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 84% identity to sit:TM1040_3167)

MetaCyc: 47% identical to L-Ala-D/L-Glu epimerase (Escherichia coli K-12 substr. MG1655)
RXN0-5228 [EC: 5.1.1.20]

Predicted SEED Role

"mandelate racemase/muconate lactonizing enzyme family protein" in subsystem Catechol branch of beta-ketoadipate pathway

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E4I5 at UniProt or InterPro

Protein Sequence (321 amino acids)

>PGA1_c30470 mandelate racemase / muconate lactonizing enzyme (Phaeobacter inhibens DSM 17395)
MQISVTPDTFKLAQVFTISRGSRTEAKVLTVRIIQDGVTGCGECVPYARYGETLESVTAE
IEGLPETFNRAELQSLLPAGAARNAVDCALWDLEAKQAGKRVWELAGLPEPKPEITAYTL
SLDSPENMQAQAAKNAHRPLLKIKLGTADDMARLEAVRAGAPEARIIVDANEGWSAEVYA
DLAPHLVRLGVDLVEQPLPAGEDAALLGMERPVPVCADESCHDRESLAALEGKYDVINIK
LDKTGGLTEALKLRDAALAQGFEVMVGCMVGSSLAMAPATLVAQGAVVTDLDGPLLLAED
REEPLDFDADGVHPPKAALWG