Protein Info for Psest_3047 in Pseudomonas stutzeri RCH2

Annotation: methionyl-tRNA synthetase/methionyl-tRNA synthetase C-terminal region/beta chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 PF09334: tRNA-synt_1g" amino acids 7 to 395 (389 residues), 519.2 bits, see alignment E=1.5e-159 TIGR00398: methionine--tRNA ligase" amino acids 7 to 535 (529 residues), 585.2 bits, see alignment E=1.3e-179 PF00133: tRNA-synt_1" amino acids 10 to 71 (62 residues), 32.9 bits, see alignment 8.1e-12 PF19303: Anticodon_3" amino acids 413 to 517 (105 residues), 29.7 bits, see alignment E=1.4e-10 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 542 to 679 (138 residues), 135.2 bits, see alignment E=1.4e-43 PF01588: tRNA_bind" amino acids 584 to 678 (95 residues), 77.8 bits, see alignment E=1.4e-25

Best Hits

Swiss-Prot: 95% identical to SYM_PSEU5: Methionine--tRNA ligase (metG) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 95% identity to psa:PST_1262)

MetaCyc: 64% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQ93 at UniProt or InterPro

Protein Sequence (680 amino acids)

>Psest_3047 methionyl-tRNA synthetase/methionyl-tRNA synthetase C-terminal region/beta chain (Pseudomonas stutzeri RCH2)
MSEARQILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKLRGNQCIYVCADDAHGSAIML
RAEKEGITPEQLIANVQAEHSGDFADFLVDFDNFHSTHSEENRELAELIYTRLRDVGHIA
TRSVTQYFDPEKGMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYEPTELKHPRSAISGA
TPVLKDSKHFFFKLPDFEAMLKQWTRGGALQESVANKIAEWLDGGLQEWDISRDAPYFGF
EIPGEPGKYFYVWLDAPIGYMASFENLCKRRPELSFDTFWSKDSTAELYHFIGKDIINFH
TLFWPAMLEGAGFRKPTAVNVHGYLTVNGQKMSKSRGTFIKARTYLDHLNPEYLRYYYAS
KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNGGVMVDANPEPELWAAF
QEAAPGIAEAYEARDFARAMREIMALADRANAWIADKAPWALNKVEGKQAEVQEICAFGV
NLFRQLVIFLKPVLPNLAAAAEQFLNVKPLRWNDHATLLANHQLNAFTPLMTRIEPAKIE
AMIEASKEDLASSEASAAPAGNGELTKQPIATEINFDAFAAVDLRIALIEKAEFVEGADK
LLRLTLDIGDAKRNVFSGIKSAYPDPGKLEGRLTLYVANLAPRKMKFGMSEGMVLAAGPG
GSEIYLLSPDNGAKPGQRVM