Protein Info for Psest_3025 in Pseudomonas stutzeri RCH2
Annotation: Cytochrome bd-type quinol oxidase, subunit 1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to CYDA_AZOVI: Cytochrome bd ubiquinol oxidase subunit 1 (cydA) from Azotobacter vinelandii
KEGG orthology group: K00425, cytochrome bd-I oxidase subunit I [EC: 1.10.3.-] (inferred from 93% identity to psa:PST_1281)MetaCyc: 70% identical to cytochrome bd-I subunit 1 (Escherichia coli K-12 substr. MG1655)
RXN0-5266 [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]; 1.11.1.- [EC: 7.1.1.7]
Predicted SEED Role
"Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-)" in subsystem Terminal cytochrome d ubiquinol oxidases or Terminal cytochrome oxidases (EC 1.10.3.-)
MetaCyc Pathways
- NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
- NADH to cytochrome bd oxidase electron transfer II (2/2 steps found)
- succinate to cytochrome bd oxidase electron transfer (2/2 steps found)
- pyruvate to cytochrome bd oxidase electron transfer (1/2 steps found)
Isozymes
Compare fitness of predicted isozymes for: 1.10.3.-
Use Curated BLAST to search for 1.10.3.- or 7.1.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GQE5 at UniProt or InterPro
Protein Sequence (546 amino acids)
>Psest_3025 Cytochrome bd-type quinol oxidase, subunit 1 (Pseudomonas stutzeri RCH2) MISESVVDLSRLQFAMTAMYHFLFVPLTLGLAFLLAIMESVYVMTGKQVYKDMTQFWGKL FGINFALGVTTGLTMEFQFGTNWAYYSHYVGDIFGAPLAIEGLMAFFLESTFIGLFFFGW DRLSKVQHLAVTWLVALGSNLSALWILIANGWMQNPVGSEFNFETMRMELVDFGALLFNP VAQVKFVHTVSAGYVTGAIFVLAISSFYLLKKRDLGFARRSFAIAAVFGLASTISVIILG DESGYEIGDVQKVKLAAIEAEWDTHPAPAGFTLFGLPNQQEMRTDYEVKIPYALGLIATR SVDEEIKGIKQLVAEHELRIRNGMLAYERLQVLRSGDKSAEAIAAFNEVKHDLGYGLLLK KYTADVVDASEEQIKLAALDTIPNVFSLFWTFRVMVAAGFLMLLLFALASWASIKRDAES KPWLLRFALFSLPLPWIAAQTGWYVAEHGRQPWSIAEVLPTHLSTSTLAAGDIWGSLIAL VAFYSLLLVIEMFLMIRFARLGPSSLHTGRYHFELEAIAASQVQRRRDAVAAPTVAKDVP PEPTAV